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Rabbit polyclonal to Human intestinal alkaline phosphatase
Predicted to work with:
Mouse, Rat, Cow
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 3 and 216 of Human intestinal alkaline phosphatase (NP_001622)
293T, A431, H1299, HeLa, Molt-4 and Raji cell lysate. HepG2 whole cell lysate. Paraffin-embedded SG xenograft
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 57 kDa.
1/100 - 1/500.
Belongs to the alkaline phosphatase family.
Information by UniProt
Alkaline phosphatase intestinal antibody
Alkaline phosphomonoesterase antibody
Western blot - Anti-Human intestinal alkaline phosphatase antibody (ab97532)
Anti-Human intestinal alkaline phosphatase antibody (ab97532) at 1/1000 dilution + Hep G2 whole cell lsyate at 30 µg
Predicted band size: 57 kDa 7.5% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human intestinal alkaline phosphatase antibody (ab97532)
Immunohistochemical analysis of paraffin-embedded SG xenograft, using ab97532 at 1/100 dilution.
This product has been referenced in:
et al. The intestinal epithelial cell differentiation marker intestinal alkaline phosphatase (ALPi) is selectively induced by histone deacetylase inhibitors (HDACi) in colon cancer cells in a Kruppel-like factor 5 (KLF5)-dependent manner. J Biol Chem 289:25306-16 (2014).
Read more (PubMed: 25037223) »
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"