Anti-Human intestinal alkaline phosphatase antibody (ab97532)

Overview

  • Product name
    Anti-Human intestinal alkaline phosphatase antibody
    See all intestinal alkaline phosphatase primary antibodies
  • Description
    Rabbit polyclonal to Human intestinal alkaline phosphatase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 3 and 216 of Human intestinal alkaline phosphatase (NP_001622)

  • Positive control
    • 293T, A431, H1299, HeLa, Molt-4 and Raji cell lysate. HepG2 whole cell lysate. Paraffin-embedded SG xenograft

Properties

Applications

Our Abpromise guarantee covers the use of ab97532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 57 kDa.
IHC-P 1/100 - 1/500.

Target

Images

  • Anti-Human intestinal alkaline phosphatase antibody (ab97532) at 1/1000 dilution + Hep G2 whole cell lsyate at 30 µg

    Predicted band size: 57 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded SG xenograft, using ab97532 at 1/100 dilution.

References

This product has been referenced in:
  • Shin J  et al. The intestinal epithelial cell differentiation marker intestinal alkaline phosphatase (ALPi) is selectively induced by histone deacetylase inhibitors (HDACi) in colon cancer cells in a Kruppel-like factor 5 (KLF5)-dependent manner. J Biol Chem 289:25306-16 (2014). WB ; Human . Read more (PubMed: 25037223) »

See 1 Publication for this product

Customer reviews and Q&As

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Small intestine)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
Small intestine
Blocking step
Normal goat serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Miriam Ayuso

Verified customer

Submitted Mar 30 2018

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (small intestine)
Loading amount
25 µg
Specification
small intestine
Gel Running Conditions
Reduced Denaturing (8 % gel)
Blocking step
3% BSA, 5% Milk, Blocking Buffer for fluorescent Western Blotting as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Username

Abcam user community

Verified customer

Submitted May 12 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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