Key features and details
- One-wash 90 minute protocol
- Sensitivity: 247.4 pg/ml
- Range: 0.5 ng/ml - 32 ng/ml
- Sample type: Cell culture extracts, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman IRAK4 ELISA Kit
See all IRAK4 kits
Intra-assay Sample n Mean SD CV% THP1 extract 8 5% Inter-assay Sample n Mean SD CV% THP1 extract 3 4%
Sample typeCell culture extracts, Tissue Extracts
Assay typeSandwich (quantitative)
Range0.5 ng/ml - 32 ng/ml
Sample specific recovery Sample type Average % Range Cell culture extracts 111 107% - 115% Tissue Extracts 101 100% - 101%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Human
Human IRAK4 ELISA Kit (ab213472) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IRAK4 protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IRAK4 with 247.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
IRAK4 is a serine/threonine protein kinase involved in the innate immune response. IRAK4 is recruited by MD88 through TLR signalling, where it phosphorylates IRAK1 and ultimately results in NF-kappa-B nuclear translocation and activation.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
PlatformPre-coated microplate (6 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Human IRAK4 Capture Antibody 1 x 600µl 10X Human IRAK4 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BC 1 x 6ml Human IRAK4 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
FunctionRequired for the efficient recruitment of IRAK1 to the IL-1 receptor complex following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Phosphorylates IRAK1.
Involvement in diseaseDefects in IRAK4 are the cause of recurrent isolated invasive pneumococcal disease type 1 (IPD1) [MIM:610799]. Recurrent invasive pneumococcal disease (IPD) is defined as two episodes of IPD occurring at least 1 month apart, whether caused by the same or different serotypes or strains. Recurrent IPD occurs in at least 2% of patients in most series, making IPD the most important known risk factor for subsequent IPD.
Defects in IRAK4 are the cause of IRAK4 deficiency (IRAK4D) [MIM:607676]. IRAK4 deficiency causes extracellular pyogenic bacterial and fungal infections in otherwise healthy children.
Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Contains 1 death domain.
Contains 1 protein kinase domain.
- Information by UniProt
- IL-1 receptor-associated kinase 4
- Interleukin 1 receptor associated kinase 4 mutant form 1
- Interleukin-1 receptor-associated kinase 4
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
Background-subtracted data values (mean +/- SD) are graphed.
The concentrations of IRAK4 were measured in duplicate and interpolated from the IRAK4 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IRAK4 concentration was determined to be 40.5 ng/mL in THP1 extract and 45.2 ng/mL in PBMC extract.
To learn more about the advantages of recombinant antibodies see here.
ab213472 has not yet been referenced specifically in any publications.