Overview

  • Product name

    Human Junctional Adhesion Molecule 1/JAM-A ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 572pg/ml 22.31 3.9%
    2 16 1145pg/ml 49.24 4.3%
    3 16 1963pg/ml 102.1 5.2%
    Inter-assay
    Sample n Mean SD CV%
    1 24 631pg/ml 35.34 5.6%
    2 24 1398pg/ml 85.28 6.1%
    3 24 2120pg/ml 156.9 7.4%
  • Sample type

    Cell culture supernatant, Serum
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    46.9 pg/ml - 3000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human Junctional Adhesion Molecule 1/JAM-A Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213800) is designed for the quantitative detection of Human JAM-A in cell culture supernatants and serum.


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for JAM-A has been pre-coated onto 96-well plates. Standards (Expression system for standard: E.coli; Immunogen sequence: S28-V238) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for JAM-A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human JAM-A amount of sample captured in plate. 

  • Notes

    Junctional adhesion molecule A/JAM-A is a protein that in humans is encoded by the F11R gene. It is mapped to 1q23.3. This gene is an immunoglobulin-like molecule that co-localizes with tight junctions in endothelium and epithelium and is also found on blood leukocytes and platelets. JAM-A plays an important role in the regulation of tight junction assembly in epithelia. In addition, it can act as a receptor for reovirus, a ligand for the integrin LFA1, involved in leukocyte transmigration and a platelet receptor. JAM-A has a non-redundant role in controlling DC motility, trafficking to lymph nodes, and activation of specific immunity.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human JAM-A coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti-Human JAM-A antibody 1 x 130µl
    Lyophilized recombinant Human JAM-A standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Seems to plays a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily.
    Contains 2 Ig-like V-type (immunoglobulin-like) domains.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Cell junction > tight junction. Cell membrane. Localized at tight junctions of both epithelial and endothelial cells.
  • Information by UniProt
  • Alternative names

    • CD 321
    • CD321
    • CD321 antigen
    • ESTM33
    • F11 receptor
    • F11R
    • JAM
    • JAM 1
    • JAM A
    • JAM-1
    • JAM-A
    • JAM1
    • JAM1_HUMAN
    • JAMA
    • JCAM
    • Jcam1
    • Junction adhesion molecule 1
    • Junction adhesion molecule, mouse, homolog of
    • Junctional adhesion molecule 1
    • Junctional adhesion molecule A
    • KAT
    • Ly106
    • PAM 1
    • PAM-1
    • PAM1
    • Platelet adhesion molecule
    • Platelet adhesion molecule 1
    • Platelet F11 receptor
    • PRO301
    • UNQ264
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human JAM-A ELISA Kit (F11R) (ab213800) Standard Curve.

Protocols

References

ab213800 has not yet been referenced specifically in any publications.

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