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The Human Kallikrein 11 (KLK11) Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213801) is designed for the quantitative measurement of Human KLK11 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for KLK11 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KLK11 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human KLK11 amount of sample captured in plate.
Kallikrein 11 is a protein that in humans is encoded by the KLK11 gene. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in two transcript variants encoding two different isoforms which are differentially expressed.
|Components||1 x 96 tests|
|ABC Diluent Buffer||1 x 12ml|
|Adhesive Plate Seal||4 units|
|Antibody Diluent Buffer||1 x 12ml|
|Anti-Human KLK11 coated Microplate (12 x 8 wells)||1 unit|
|Avidin-Biotin-Peroxidase Complex (ABC)||1 x 130µl|
|Biotinylated KLK11 Antibody||1 x 130µl|
|Human KLK11 Standard (Lyophilized)||2 vials|
|Sample Diluent Buffer||1 x 30ml|
|TMB Color Developing Agent||1 x 10ml|
|TMB Stop Solution||1 x 10ml|
Our Abpromise guarantee covers the use of ab213801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab213801 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"