• Product name

    Human Kallikrein 11 ELISA Kit
  • Detection method

  • Precision

    Sample n Mean SD CV%
    1 16 1399pg/ml 68.55 4.9%
    2 16 3520pg/ml 204.16 5.8%
    3 16 6635pg/ml 364.92 5.5%
    Sample n Mean SD CV%
    1 24 1575pg/ml 92.92 5.9%
    2 24 4236pg/ml 279.57 6.6%
    3 24 6751pg/ml 398.3 5.9%
  • Sample type

    Cell culture supernatant, Serum, Cell Lysate, Heparin Plasma, EDTA Plasma, Tissue Homogenate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    156 pg/ml - 10000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human Kallikrein 11 (KLK11) Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213801) is designed for the quantitative measurement of Human KLK11 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

    The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for KLK11 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KLK11 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human KLK11 amount of sample captured in plate.

  • Notes

    Kallikrein 11 is a protein that in humans is encoded by the KLK11 gene. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in two transcript variants encoding two different isoforms which are differentially expressed.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)


  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human KLK11 coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated KLK11 Antibody 1 x 130µl
    Human KLK11 Standard (Lyophilized) 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues.
  • Tissue specificity

    Expressed in brain, skin and prostate. Isoform 1 is expressed preferentially in brain. Isoform 2 is expressed in prostate. Present in seminal plasma at concentrations ranging from 2 to 37 microg/mL (at protein level).
  • Sequence similarities

    Belongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.
  • Post-translational

    About 40% of KLK11 is inactivated by internal cleavage after Arg-188. This proteolytic inactivation may be effected by plasminogen.
  • Cellular localization

  • Information by UniProt
  • Alternative names

    • Hippostasin
    • hK11
    • Kallikrein 11
    • Kallikrein 11 isoform 1 preproprotein
    • Kallikrein 11 isoform 2 precursor
    • Kallikrein 11 precursor
    • Kallikrein related peptidase 11
    • Kallikrein-11 inactive chain 2
    • KLK 11
    • Klk11
    • KLK11_HUMAN
    • MGC33060
    • Protease serine 20 trypsin like
    • Protease serine trypsin like
    • PRSS20
    • Serine protease 20
    • TLSP
    • Trypsin like protease
    • Trypsin-like protease
    see all
  • Database links


Our Abpromise guarantee covers the use of ab213801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Human KLK11 ELISA Kit (ab213801) Standard Curve.



ab213801 has not yet been referenced specifically in any publications.

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