Overview

  • Product name

    Human Kallikrein 12 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 1409pg/ml 73.26 5.2%
    2 16 3882pg/ml 213.51 5.5%
    3 16 6756pg/ml 405.36 6%
    Inter-assay
    Sample n Mean SD CV%
    1 24 1523pg/ml 89.85 5.9%
    2 24 4129pg/ml 251.86 6.1%
    3 24 6526pg/ml 443.76 6.8%
  • Sample type

    Cell culture supernatant, Serum, Cell Lysate, Heparin Plasma, EDTA Plasma, Tissue Homogenate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    156 pg/ml - 10000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human Kallikrein 12 (KLK12) Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213802) is designed for the quantitative measurement of Human KLK12 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).


    The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for KLK12 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KLK12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human KLK12 amount of sample captured in plate.

  • Notes

    Kallikrein-12 is a protein that in humans is encoded by the KLK12 gene. It belongs to the kallikrein subgroup of serine proteases, which have diverse physiologic functions in many tissues. KLK12 is expressed primarily in salivary gland, stomach, uterus, trachea, prostate, thymus, lung, colon, brain, breast, and thyroid gland and at lower levels in testis, pancreas, small intestine, and spinal cord. The expression of KLK12 is downregulated at the mRNA level in breast cancer tissues and is upregulated by steroid hormones in breast and prostate cancer cell lines.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human KLK12 coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated KLK12 Antibody 1 x 130µl
    Human KLK12 Standard (Lyophilized) 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Relevance

    The human tissue Kallikrein gene family encodes 15 serine proteases. All Kallikreins share structural similarities including cysteine residues, a catalytic triad of His, Asp, and Ser residues, typically five coding exons, and varied intron phases. Kallikreins are predominantly secreted as inactive zymogens prior to activation by cleavage of an N-terminal peptide, and all function extracellularly. Kallikreins can be activated autocatalytically, via other Kallikreins, or additional proteases. While structurally similar, Kallikrein family members have distinct functions and have key roles in many physiological and pathological processes. Many human tissue Kallikreins also show promise as cancer biomarkers, which may facilitate earlier detection and characterization of many forms of cancer. Kallikrein 12, also known as Kallikrein-like5 (KLK-L5), is a trypsin-like serine proteinase. It was discovered by cloning the kallikrein locus on chromosome 19, and was found to be similar to the other kallikrein family genes. It is produced by a number of tissues, including pancreas, salivary gland, breast, prostate, uterus, lung, thymus, thyroid, brain, stomach and colon. Kallikrein 12 message has been reported to be decreased in breast cancer. Different splice variants of kallikrein-12 have been identified; a 254 amino acid form, 248 amino acids form, and a truncated 111 amino acid form.
  • Cellular localization

    Secreted
  • Alternative names

    • DKFZp686H1078
    • Kallikrein like protein 5
    • Kallikrein related peptidase 12
    • Kallikrein12
    • KLK 12
    • KLK L5
    • KLK12
    • KLKL5
    • MGC42603
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human KLK12 ELISA Kit (ab213802) Standard Curve.

Protocols

References

ab213802 has not yet been referenced specifically in any publications.

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