Overview

  • Product name

    Human Kallikrein 8 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 1468pg/ml 67.53 4.6%
    2 16 3882pg/ml 260.1 6.7%
    3 16 6352pg/ml 349.4 5.5%
    Inter-assay
    Sample n Mean SD CV%
    1 24 1625pg/ml 95.9 5.9%
    2 24 4190pg/ml 301.7 7.2%
    3 24 6526pg/ml 417.7 6.4%
  • Sample type

    Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Tissue Homogenate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    156 pg/ml - 10000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human Kallikrein 8 (KLK8) Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213804) is designed for the quantitative measurement of Human Kallikrein-8 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).


    The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Kallikrein-8 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Kallikrein-8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Kallikrein-8 amount of sample captured in plate.

  • Notes

    Kallikrein-8 is a protein that in humans is encoded by the KLK8 gene. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in four transcript variants encoding four different isoforms. The isoforms exhibit distinct patterns of expression that suggest roles in brain plasticity and ovarian cancer.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human KLK8 coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated KLK8 Antibody 1 x 130µl
    Human KLK8 Standard (Lyophilized) 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Serine protease which is capable of degrading a number of proteins such as casein, fibrinogen, kininogen, fibronectin and collagen type IV. Also cleaves L1CAM in response to increased neural activity. Induces neurite outgrowth and fasciculation of cultured hippocampal neurons. Plays a role in the formation and maturation of orphan and small synaptic boutons in the Schaffer-collateral pathway, regulates Schaffer-collateral long-term potentiation in the hippocampus and is required for memory acquisition and synaptic plasticity. Involved in skin desquamation and keratinocyte proliferation. Plays a role in the secondary phase of pathogenesis following spinal cord injury.
  • Tissue specificity

    Isoform 1 is predominantly expressed in the pancreas. Isoform 2 is expressed in adult brain and hippocampus. Isoform 1 and isoform 2 are found in fetal brain and placenta. Detected in salivary gland, uterus, thymus, breast, testis and kidney but not in spleen, liver, lung or normal ovarian tissue. Displays an 11.5-fold increase in Alzheimer disease hippocampus compared to controls and is overexpressed in some ovarian carcinomas. Expressed at low levels in normal skin while high levels are found in psoriasis vulgaris, seborrheic keratosis, lichen planus and squamous cell carcinoma skin samples. Expressed in the keratinocytes.
  • Sequence similarities

    Belongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.
  • Cellular localization

    Secreted. Cytoplasm. Shows a cytoplasmic distribution in the keratinocytes.
  • Information by UniProt
  • Alternative names

    • Brain serine protease 1
    • BSP1
    • hK8
    • HNP
    • HPN
    • Kallikrein 8 (neuropsin/ovasin)
    • Kallikrein related peptidase 8
    • Kallikrein-8
    • Kallikrein8
    • KLK 8
    • Klk8
    • KLK8 protein type 1
    • KLK8 protein type 2
    • KLK8_HUMAN
    • Neuropsin
    • neuropsin type 1
    • Neuropsin type 1, included
    • Neuropsin type 2
    • Neuropsin type 2, included
    • Neuropsin, mouse, homolog of
    • NP
    • NRPN
    • Ovasin
    • PRO322
    • Protease serine 19
    • PRSS 19
    • PRSS19
    • Serine protease 19
    • Serine protease kallikrein
    • Serine protease kallikrein/ovasin/neuropsin
    • Serine protease TADG 14
    • Serine protease TADG-14
    • Serine protease TADG14
    • TADG 14
    • TADG14
    • Tumor associated differentially expressed gene 14
    • Tumor associated differentially expressed gene 14 protein
    • Tumor-associated differentially expressed gene 14 protein
    • UNQ283
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human Kallikrein-8 ELISA Kit (ab213804) Standard Curve.

Protocols

References

ab213804 has not yet been referenced specifically in any publications.

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