Recombinant Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)
![Western blot - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)](https://www.abcam.com/ps/products/134/ab134929/Images/ab134929-149104-anti-human-kappa-chain-antibody-epr5539-71-8-western-blot.jpg "Western blot - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5539-71-8] to Human Kappa Chain
- Suitable for: ICC, WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Human Kappa Chain antibody [EPR5539-71-8]
See all Human Kappa Chain primary antibodies -
Description
Rabbit monoclonal [EPR5539-71-8] to Human Kappa Chain -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WB, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human tonsil, Human spleen, Human thymus, and Human plasma lysates. B cell lymphoma tissue. Human tonsil tissue. ICC: Raji cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5539-71-8 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 647 Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab200631)
- Biotin Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab201490)
- Anti-Human Kappa Chain antibody [EPR5539-71-8] - BSA and Azide free (ab211641)
- Rabbit monoclonal [EPR5539-71-8] Anti-Human kappa light chain (Alexa Fluor® 647) (ab236479)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134929 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
1/250 - 1/500.
|
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| WB |
1/1000 - 1/10000. Predicted molecular weight: 49 kDa.
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| Notes |
|---|
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ICC
1/250 - 1/500. |
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WB
1/1000 - 1/10000. Predicted molecular weight: 49 kDa. |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Relevance
Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 3514 Human
- Omim: 147200 Human
- SwissProt: P01834 Human
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Alternative names
- HCAK 1 antibody
- HCAK1 antibody
- Ig kappa chain C region antibody
see all
Images
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Western blot - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)All lanes : Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929) at 1/1000 dilution
Lane 1 : Human tonsil lysate
Lane 2 : Human spleen lysate
Lane 3 : Human thymus lysate
Lane 4 : Human plasma lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 49 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)](https://www.abcam.com/ps/products/134/ab134929/Images/ab134929-149103-anti-human-kappa-chain-antibody-epr5539-71-8-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)Immunohistochemical analysis of paraffin-embedded B cell lymphoma tissue labelling IgM with ab134929 at 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunocytochemistry - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)](https://www.abcam.com/ps/products/134/ab134929/Images/ab134929-6-anti-human-kappa-chain-antibody-epr5539-immunocytochemistry-raji-human.jpg)
Immunocytochemistry - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)Immunocytochemistry analysis of Raji (Human Burkitt's lymphoma B lymphocyte? labeling Human Kappa Chain with purified ab134929 at 1/100 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.32 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control: PBS instead of the primary antibody. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)](https://www.abcam.com/ps/products/134/ab134929/Images/ab134929-149102-anti-human-kappa-chain-antibody-epr5539-71-8-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling IgM with ab134929 at 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)](https://www.abcam.com/ps/products/134/ab134929/Images/ab134929-149101-anti-human-kappa-chain-antibody-epr5539-71-8-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)Immunohistochemical analysis using ab134929 showing positive staining in Normal colon, lymphocytes tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)](https://www.abcam.com/ps/products/134/ab134929/Images/ab134929-149100-anti-human-kappa-chain-antibody-epr5539-71-8-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)Immunohistochemical analysis using ab134929 showing positive staining in Normal stomach, lymphocytes tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)](https://www.abcam.com/ps/products/134/ab134929/Images/ab134929-7-benefits-of-recombinant-antibodies.png)
Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab134929 has been referenced in 1 publication.
- Jing Z et al. Expression of immunoglobulin G in human podocytes, and its role in cell viability and adhesion. Int J Mol Med 41:3296-3306 (2018). PubMed: 29512722