RecombinantRabMAb

Recombinant Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)

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Western blot - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)
  • Immunocytochemistry - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)
  • Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5539-71-8] to Human Kappa Chain
  • Suitable for: ICC, WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Biotin Carrier Free

Overview

  • Product name

    Anti-Human Kappa Chain antibody [EPR5539-71-8]
    See all Human Kappa Chain primary antibodies

  • Description

    Rabbit monoclonal [EPR5539-71-8] to Human Kappa Chain

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, IHC-Pmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human tonsil, Human spleen, Human thymus, and Human plasma lysates. B cell lymphoma tissue. Human tonsil tissue. ICC: Raji cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR5539-71-8

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
1/250 - 1/500.
WB
1/1000 - 1/10000. Predicted molecular weight: 49 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
ICC
1/250 - 1/500.
WB
1/1000 - 1/10000. Predicted molecular weight: 49 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Application notes
Is unsuitable for IP.

Target

  • Relevance

    Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin.

  • Cellular localization

    Cytoplasmic

  • Database links

    • Alternative names

      • HCAK 1 antibody
      • HCAK1 antibody
      • Ig kappa chain C region antibody
      • IGKC antibody
      • IMMUNOGLOBULIN InV antibody
      • Immunoglobulin kappa constant antibody
      • Immunoglobulin kappa constant region antibody
      • Immunoglobulin kappa light chain antibody
      • KAPPA-CHAIN DEFICIENCY antibody
      • Km antibody
      • MGC11157 antibody
      • MGC62011 antibody
      • MGC72072 antibody
      • MGC88770 antibody
      • MGC88771 antibody
      • MGC88809 antibody
      see all

    Images

    • Western blot - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)
      Western blot - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)
      All lanes : Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929) at 1/1000 dilution

      Lane 1 : Human tonsil lysate
      Lane 2 : Human spleen lysate
      Lane 3 : Human thymus lysate
      Lane 4 : Human plasma lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 49 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)

      Immunohistochemical analysis of paraffin-embedded B cell lymphoma tissue labelling IgM with ab134929 at 1/250 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunocytochemistry - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)
      Immunocytochemistry - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)

      Immunocytochemistry analysis of Raji (Human Burkitt's lymphoma B lymphocyte? labeling Human Kappa Chain with purified ab134929 at 1/100 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.32 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
      Negative control: PBS instead of the primary antibody.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)

      Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling IgM with ab134929 at 1/250 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)

      Immunohistochemical analysis using ab134929 showing positive staining in Normal colon, lymphocytes tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)

      Immunohistochemical analysis using ab134929 showing positive staining in Normal stomach, lymphocytes tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)
      Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)

    References (1)

    Publishing research using ab134929? Please let us know so that we can cite the reference in this datasheet.

    ab134929 has been referenced in 1 publication.

    • Jing Z  et al. Expression of immunoglobulin G in human podocytes, and its role in cell viability and adhesion. Int J Mol Med 41:3296-3306 (2018). PubMed: 29512722

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