Overview

  • Product name

    Anti-Human Kappa Chain antibody [SB81a] (HRP)
    See all Human Kappa Chain primary antibodies
  • Description

    Mouse monoclonal [SB81a] to Human Kappa Chain (HRP)
  • Host species

    Mouse
  • Conjugation

    HRP
  • Specificity

    ab79115 reacts with human kappa light chains.
  • Tested applications

    Suitable for: ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    The details of the immunogen for this antibody are not available.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: None
    Constituents: 50% PBS, 50% Glycerol, pH 7.4
  • Concentration information loading...
  • Purity

    Ascites
  • Purification notes

    Purified from ascites.
  • Clonality

    Monoclonal
  • Clone number

    SB81a
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab79115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000 - 1/2000.
ICC/IF Use at an assay dependent dilution.

Target

  • Relevance

    Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin.
  • Cellular localization

    Cytoplasmic
  • Database links

  • Alternative names

    • HCAK 1 antibody
    • HCAK1 antibody
    • Ig kappa chain C region antibody
    • IGKC antibody
    • IMMUNOGLOBULIN InV antibody
    • Immunoglobulin kappa constant antibody
    • Immunoglobulin kappa constant region antibody
    • Immunoglobulin kappa light chain antibody
    • KAPPA-CHAIN DEFICIENCY antibody
    • Km antibody
    • MGC11157 antibody
    • MGC62011 antibody
    • MGC72072 antibody
    • MGC88770 antibody
    • MGC88771 antibody
    • MGC88809 antibody
    see all

Images

  • ELISA plate was coated with purified human IgGκ, IgAκ, IgMκ, IgGλ, IgAλ, and IgMλ.  Immunoglobulins were detected with serially diluted Mouse Anti-Human Kappa Chain antibody (HRP) ab79115.

References

This product has been referenced in:

  • Scheid JF  et al. HIV-1 antibody 3BNC117 suppresses viral rebound in humans during treatment interruption. Nature 535:556-60 (2016). Read more (PubMed: 27338952) »
  • Caskey M  et al. Viraemia suppressed in HIV-1-infected humans by broadly neutralizing antibody 3BNC117. Nature 522:487-91 (2015). Read more (PubMed: 25855300) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

We do not have expiration dates or shelf lives for our products. We guarantee our products for a year so if within this year you have any problems, please do not hesitate to contact us and we will be happy to help.
Indeed ab79115 should be stored at 4ºC as stated on the datasheet. Given that this antibody is conjugated to HRP which is an enzyme, it should not be frozen. Freezing and thawing will reduce enzymatic activity in addition to affecting the antibody binding capacity.

Read More

Answer


The lab let me know that they recently tested the monoclonal Anti-Human Kappa (ab79115) by ELISA and determined that it exhibits minimal cross reactivity (<1%) with various mouse immunoglobulins.

Read More

Question
Answer

Thank you for contacting us. I have checked with the lab and found the product you were looking for. It is already in the catalog as ab99832. https://www.abcam.com/index.html?datasheet=99832 It is currently in stock. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up