Anti-Human Kappa Chain antibody [TB-28] - BSA and Azide free (ab235128)
Key features and details
- Mouse monoclonal [TB-28] to Human Kappa Chain - BSA and Azide free
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Human Kappa Chain antibody [TB-28] - BSA and Azide free
See all Human Kappa Chain primary antibodies -
Description
Mouse monoclonal [TB-28] to Human Kappa Chain - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein corresponding to Human Human Kappa Chain. (Human IgA, kappa).
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Positive control
- Flow cyt: Human peripheral blood mononuclear cells. IHC-P: Human tonsil tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
TB-28 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Conjugation kits
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab235128 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use a concentration of 1 µg/ml.
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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Flow Cyt
Use a concentration of 1 µg/ml. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 3514 Human
- Omim: 147200 Human
- SwissProt: P01834 Human
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Alternative names
- HCAK 1 antibody
- HCAK1 antibody
- Ig kappa chain C region antibody
see all
Images
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Human peripheral blood mononuclear cells stained with ab235128 (right) or mouse IgG1κ (ab170190) isotype (left). Human peripheral blood mononuclear cells were incubated for 30 min on ice in 1x PBS containing 10 µg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab235128) or mouse IgG1κ (ab170190) (1x106 in 100 µl at 1 µg/ml) for 30 min on ice.
The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150177) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD19 antibody.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.
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IHC image of human Kappa chain staining in a section of formalin-fixed paraffin-embedded normal human tonsil* performed on a Leica BOND<™> system using the standard Protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab235128, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (2)
ab235128 has been referenced in 2 publications.
- Kubagawa H et al. Immunoglobulin heavy-chain switching in pre-B leukaemias. Nature 301:340-2 (1983). PubMed: 6185849
- Kubagawa H et al. Immunoglobulin isotype expression of normal pre-B cells as determined by immunofluorescence. J Clin Immunol 2:264-9 (1982). PubMed: 6815220