Key features and details
- Sensitivity: 1.8304 ng/ml
- Range: 6.25 ng/ml - 200 ng/ml
- Sample type: Other biological fluids, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman Kappa ELISA Kit
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 10%
Sample typeSerum, Plasma, Other biological fluids
Assay typeSandwich (quantitative)
Range6.25 ng/ml - 200 ng/ml
Sample specific recovery Sample type Average % Range Serum > 85 % - %
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam's Kappa Human ELISA Kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Kappa in Human serum and plasma samples.
In this assay the Kappa present in samples reacts with the anti-Kappa antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-Kappa antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Kappa. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Kappa in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Kappa in the test sample. The quantity of Kappa in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 100X HRP-conjugated anti-Human Kappa antibody 1 x 150µl 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml Chromogen Substrate Solution 1 x 12ml Human Kappa Calibrator (Lyophilized) 1 vial Human Kappa ELISA Microplate 1 unit Stop Solution 1 x 12ml
RelevanceImmunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.
- Ig kappa chain C region
ab157709 has been referenced in 1 publication.
- Mosleth EF et al. Cerebrospinal fluid proteome shows disrupted neuronal development in multiple sclerosis. Sci Rep 11:4087 (2021). PubMed: 33602999