Recombinant Anti-Human Kappa Light Chain antibody [RM126] (ab195576)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM126] to Human Kappa Light Chain
- Suitable for: IHC-P, ELISA
- Reacts with: Human
Overview
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Product name
Anti-Human Kappa Light Chain antibody [RM126] -
Description
Rabbit monoclonal [RM126] to Human Kappa Light Chain -
Host species
Rabbit -
Specificity
ab195576 reacts to the kappa light chain of human immunoglobulins. No cross reactivity with the lambda light chain, mouse IgG, rat IgG, or goat IgG. -
Tested applications
Suitable for: IHC-P, ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human Human Kappa Light Chain. Human IgG purified from Human plasma.
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Positive control
- Human tonsil tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 48% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
ab195576 was purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM126 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab195576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 0.5 - 2 µg/ml.
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ELISA |
Use a concentration of 0.05 - 0.2 µg/ml.
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Notes |
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IHC-P
Use a concentration of 0.5 - 2 µg/ml. |
ELISA
Use a concentration of 0.05 - 0.2 µg/ml. |
Target
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Cellular localization
Secreted -
Database links
- SwissProt: Q6P5S8 Human
Images
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The wells were coated with ab195576 at 0.5µg/ml at 50µl/well overnight at 4°C, followed by a 5% BSA blocking step for 2h RT. Human IgG (ab98981) was then added starting at 10 µg/ml and plasma/serum at 1:10 and gradually diluted 1:4, 50µl/well for 2h. Ab201484 was then added at 1:10,000 dilution, 50µl/well for 2h. A HRP-streptavidin (ab7403) was used at 1:10,000 dilution for 1h.
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Immunohistochemical analysis of Human tonsil tissue labeling Human IgG Kappa Light Chain with ab195576 at 2 µg/ml.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab195576 has not yet been referenced specifically in any publications.