Key features and details
- Mouse monoclonal [SPM508] to Human Kappa Light Chain
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Human Kappa Light Chain antibody [SPM508]
See all Human Kappa Light Chain primary antibodies
DescriptionMouse monoclonal [SPM508] to Human Kappa Light Chain
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Tissue, cells or virus corresponding to Human Kappa Light Chain. B-lymphoma cells.
- WB: Human spleen lysate. IHC-P: Human tonsil, B-cell lymphoma, colon and HK lymphoma tissue.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact firstname.lastname@example.org.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified by Protein A/G from TCS.
Our Abpromise guarantee covers the use of ab227744 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Boil tissue section in citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.
|WB||1/25. Predicted molecular weight: 12 kDa.|
- SwissProt: Q6P5S8 Human
Anti-Human Kappa Light Chain antibody [SPM508] (ab227744) at 1/25 dilution + Human spleen lysate
Developed using the ECL technique.
Predicted band size: 12 kDa
Formalin-fixed, paraffin-embedded human tonsil tissue stained for Human Kappa Light Chain with ab227744 at 1/200 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human B-cell lymphoma tissue stained for Human Kappa Light Chain with ab227744 at 1/200 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human colon tissue stained for Human Kappa Light Chain with ab227744 at 1/200 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human HK lymphoma tissue stained for Human Kappa Light Chain with ab227744 at 1/200 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227744 has not yet been referenced specifically in any publications.