Overview

  • Product name

    Anti-Human Kappa Light Chain antibody [SPM508]
    See all Human Kappa Light Chain primary antibodies
  • Description

    Mouse monoclonal [SPM508] to Human Kappa Light Chain
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human Kappa Light Chain. B-lymphoma cells.

  • Positive control

    • WB: Human spleen lysate. IHC-P: Human tonsil, B-cell lymphoma, colon and HK lymphoma tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.6
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified by Protein A/G from TCS.
  • Clonality

    Monoclonal
  • Clone number

    SPM508
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200.

Boil tissue section in citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

WB 1/25. Predicted molecular weight: 12 kDa.

Target

Images

  • Anti-Human Kappa Light Chain antibody [SPM508] (ab227744) at 1/25 dilution + Human spleen lysate

    Developed using the ECL technique.

    Predicted band size: 12 kDa

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for Human Kappa Light Chain with ab227744 at 1/200 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human B-cell lymphoma tissue stained for Human Kappa Light Chain with ab227744 at 1/200 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human colon tissue stained for Human Kappa Light Chain with ab227744 at 1/200 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human HK lymphoma tissue stained for Human Kappa Light Chain with ab227744 at 1/200 dilution in immunohistochemical analysis.

References

ab227744 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227744.
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