Overview

  • Product name

    Human KIM1 ELISA Kit (TIM-1)
    See all TIM 1 kits
  • Detection method

    Colorimetric
  • Sample type

    Urine
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    1.279 pg/ml
  • Range

    7.813 pg/ml - 500 pg/ml
  • Assay time

    2h 0m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The human KIM1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is a complete kit for the quantitative measurement of KIM1 protein in urine.


    The ELISA kit is ultra sensitive, reliably measuring as little as 1.2 pg/ml of Kim1. This enables the use of reduced input sample and results in reduced matrix interference. It is high throughput with results in <2 hours from up to 38 samples in duplicate. The kit uses a monoclonal antibody to KIM-1 immobilized on a microtiter plate to bind the KIM-1 in the standards or sample. After a short incubation the excess sample or standard is washed out and a biotinylated monoclonal antibody to KIM-1 is added. This antibody binds to the KIM-1 captured on the plate. After a short incubation the excess antibody is washed out and Streptavidin conjugated to Horseradish peroxidase is added, which binds to the biotin on the monoclonal antibody. The plate is then incubated. Once the incubation is complete, excess conjugate is washed out and TMB substrate solution is added. An HRP-catalyzed reaction generates a blue color in the solution. Stop solution is added to stop the substrate reaction. The resulting yellow color is read at 450 nm. The amount of signal is directly proportional to the level of KIM-1 in the sample.


    Kim1 is an early biomarker for kidney injury or disease. It is the most highly upregulated protein in the proximal tubule of the injured kidney. It exists in very low levels in normal kidneys but when upregulated during injury, it is detectable in urine in a wide variety of human diseases. KIM-1 is a potential biomarker for renal injury, which would suggest it has great importance in various kidney diseases and disorders such as chronic kidney disease, as well as acute tubular necrosis and acute kidney failure. 

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    Assay Buffer 1 x 60ml
    KIM-1 coated microplate (12x 8 well strips) 1 unit
    KIM-1 Conjugate 1 x 10ml
    KIM-1 Detector Antibody 1 x 10ml
    KIM-1 Standard (25ng/mL) 1 x 0.5ml
    Plate Sealer 3 units
    Stop Solution 2 1 x 10ml
    TMB Substrate 1 x 10ml
    Wash Buffer Concentrate 1 x 100ml
  • Research areas

  • Function

    May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair.
  • Tissue specificity

    Widely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily. TIM family.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Alternative names

    • CD365
    • HAVCR
    • HAVCR 1
    • HAVcr-1
    • Havcr1
    • Hepatitis A virus cellular receptor 1
    • Kidney injury molecule 1
    • KIM 1
    • KIM-1
    • T cell immunoglobin domain and mucin domain protein 1
    • T cell immunoglobulin mucin family member 1
    • T cell immunoglobulin mucin receptor 1
    • T-cell immunoglobulin and mucin domain-containing protein 1
    • T-cell membrane protein 1
    • TIM
    • TIM-1
    • TIM1
    • TIMD 1
    • TIMD-1
    • TIMD1
    • TIMD1_HUMAN
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab235081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

Protocols

References

ab235081 has not yet been referenced specifically in any publications.

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