Key features and details
- Suitable for: Blocking
Product nameHuman KMT4 / Dot1L peptide
See all KMT4 / Dot1L proteins and peptides
Purity70 - 90% by HPLC.
Our Abpromise guarantee covers the use of ab71337 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-KMT4 / Dot1L antibody (ab64077)
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
- Disrupter of telomere silencing protein 1
- DOT 1
FunctionHistone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA.
Sequence similaritiesBelongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.
Contains 1 DOT1 domain.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab71337 has been referenced in 1 publication.
- Ooga M et al. Involvement of DOT1L in the remodeling of heterochromatin configuration during early preimplantation development in mice. Biol Reprod 89:145 (2013). Blocking . PubMed: 24132959