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  1. Link

    human-ku70-peptide-ab92421.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair Non Homol. End Joining
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Human Ku70 peptide (ab92421)

  • Datasheet
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Abpromise

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Key features and details

  • Suitable for: Blocking

Description

  • Product name

    Human Ku70 peptide
    See all Ku70 proteins and peptides
  • Purity

    70 - 90% by HPLC.

  • Animal free

    No
  • Nature

    Synthetic
    • Species

      Human

Associated products

  • Corresponding Antibody

    • Anti-Ku70 antibody (ab83501)

Specifications

Our Abpromise guarantee covers the use of ab92421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-Ku70 antibody (ab83501)

  • Form

    Liquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names

    • 5''-deoxyribose-5-phosphate lyase Ku70
    • 5''-dRP lyase Ku70
    • 70 kDa subunit of Ku antigen
    • ATP dependent DNA helicase 2 subunit 1
    • ATP dependent DNA helicase II 70 kDa subunit
    • ATP-dependent DNA helicase 2 subunit 1
    • ATP-dependent DNA helicase II 70 kDa subunit
    • CTC box binding factor 75 kDa subunit
    • CTC box-binding factor 75 kDa subunit
    • CTC75
    • CTCBF
    • DNA repair protein XRCC6
    • G22P1
    • Ku 70
    • Ku autoantigen p70 subunit
    • Ku autoantigen, 70kDa
    • Ku p70
    • Ku70
    • Ku70 DNA binding component of DNA-dependent proteinkinase complex (thyroid autoantigen 70 kDa
    • Kup70
    • Lupus Ku autoantigen protein p70
    • ML8
    • Thyroid autoantigen
    • Thyroid autoantigen 70kD (Ku antigen)
    • Thyroid lupus autoantigen
    • Thyroid lupus autoantigen p70
    • Thyroid-lupus autoantigen
    • TLAA
    • X ray repair complementing defective repair in Chinese hamster cells 6
    • X-ray repair complementing defective repair in Chinese hamster cells 6
    • X-ray repair cross-complementing protein 6
    • XRCC 6
    • Xrcc6
    • XRCC6_HUMAN
    see all
  • Function

    Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
  • Sequence similarities

    Belongs to the ku70 family.
    Contains 1 Ku domain.
    Contains 1 SAP domain.
  • Developmental stage

    Expression does not increase during promyelocyte differentiation.
  • Post-translational
    modifications

    Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair.
  • Cellular localization

    Nucleus. Chromosome.
  • Target information above from: UniProt accession P12956 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab92421? Please let us know so that we can cite the reference in this datasheet.

    ab92421 has not yet been referenced specifically in any publications.

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