Overview

  • Product name
    Human Lactate Dehydrogenase B / LDH-B ELISA Kit
    See all Lactate Dehydrogenase B/LDH-B kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    HeLa extract 5 1.9%
    Inter-assay
    Sample n Mean SD CV%
    HeLa extract 3 2.7%
  • Sample type
    Cell culture supernatant, Serum, Tissue Extracts, Cell Lysate, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    8 pg/ml
  • Range
    0.312 ng/ml - 20 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 105 101% - 108%
    Cell culture media 108 108% - 109%
    Heparin Plasma 104 102% - 105%
    EDTA Plasma 110 107% - 115%
    Citrate Plasma 110 107% - 114%
    Extraction Buffer 98 97% - 100%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Lactate Dehydrogenase B / LDH-Bin vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Lactate Dehydrogenase B / LDH-B protein in Human serum, plasma, cell culture supernatant, cell and tissue extracts.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm.Optionally,instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


     


    Sensitivity:


    Samples diluted in Sample Diluent NS - 8 pg/mL


    Samples diluted in 1X Cell Extraction Buffer PTR - 212 pg/mL

  • Notes

    Functional lactate dehydrogenase are homo or hetero tetramers composed of M and H protein subunits encoded by the LDH-A and LDH-B genes, respectively. The major isozymes of skeletal muscle and liver has four muscle (M) subunits; while the main isozymes for heart muscle contains four (H) subunits. The other variants contain both types of subunits. LDHB converts pyruvate to lactate under low oxygen conditions, while in the LDH-A converts lactate into pyruvate. Elevated levels of LDH indicate tissue breakdown in hemolysis. LDH can be used as a marker of myocardial infarction, peaking at days 3 and lasting through day 10. Other disorders indicated by elevated LDH include cancer, meningitis, encephalitis, acute pancreatitis, and HIV. Defects in LDHB are a cause of hereditary LDH-B deficiency; however LDH-B deficiency is usually asymptomatic.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab183367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • ELISA Protocol Summary
  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Raw data in duplicate measurements are plotted. Background is represented as the dotted black line.

  • Raw data in duplicate measurements are plotted. Background is represented as the dotted black line.

  • Interpolated values of LDHB are plotted for the indicated cell lines based on an extract load of 20 µg/mL.

  • The concentrations of LDHB were interpolated from the LDHB standard curve and corrected for sample dilution. The mean LDHB concentration was determined to be 197 ng/mL in plasma (heparin) and 173 ng/mL in serum.

  • Serum from 10 apparently healthy male donors was measured in triplicate for LDHB. The mean LDHB concentration was determined to be 161 ng/mL with a range of 56 – 226 ng/mL.

Protocols

References

ab183367 has not yet been referenced specifically in any publications.

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