• Product name
    Human Lactoferrin ELISA Kit (HLF2)
    See all Lactoferrin kits
  • Detection method
  • Precision
    Sample n Mean SD CV%
    Overall 4.3%
    Sample n Mean SD CV%
    Overall 9.5%
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    = 0.35 ng/ml
  • Range
    0.625 ng/ml - 40 ng/ml
  • Recovery

    = 102 %

  • Assay time
    4h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Lactoferrin (HLF2) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Lactoferrin levels in Human cell culture supernatants, serum, urine, saliva, milk, cerebrospinal fluid and plasma.

    A Lactoferrin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Lactoferrin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Lactoferrin captured in plate.

    Get results in 90 minutes with Human Lactoferrin ELISA Kit (ab200015) from our SimpleStep ELISA® range.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


Associated products


Our Abpromise guarantee covers the use of ab108882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.


  • Lactoferrin measured in biological fluids with background signal subtracted (duplicates +/- SD).
  • Representative Standard Curve Using ab108882.



This product has been referenced in:
  • Vargas Buonfiglio LG  et al. Airway surface liquid from smokers promotes bacterial growth and biofilm formation via iron-lactoferrin imbalance. Respir Res 19:42 (2018). Read more (PubMed: 29524964) »
  • Druhan LJ  et al. Leucine Rich a-2 Glycoprotein: A Novel Neutrophil Granule Protein and Modulator of Myelopoiesis. PLoS One 12:e0170261 (2017). ELISA ; Human . Read more (PubMed: 28081565) »

See all 6 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.

The concentration of standard in the lot you received is 40 ng/ml. The standard concentration is lot specific so be sure to check the vial each time you order a kit, or call us if the label is missing. Read More

Thanks for your enquiry.

I did some calculations and it seems if one starts with 5 ul milk sample and dilutes it in 6 successive 10-fold dilutions, where the final dilution is 50ul of a 100,000-fold dilution, there would be sufficient buffer ...

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Thank you for your interest. We do not have lactoferrin assay kits with longer shelf lives, but the 6 month expiration that is specifiedis very conservative. We are confidentthe kit will be effective, assuming proper storage, within that period.

Thank you for your enquiry.

I can confirm that any publications that we are aware of will be listed on the datasheet. We have a dedicated team who spend time reviewing the literature to find articles that have used our products. However, in ...

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Thank you for contacting us.

The cross reactivity with bovine lactoferrin is less than 1%.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you very much for your email.

We have never tested equine samples. We have tested canine, bovine, monkey, mouse, rat, swine, and rabbit samples. See table below.

Species % Cross Reactivity
Canine 1%
Bovine 0.5%

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Thank you for contacting us. Unfortunately, the antibodies mentioned are not suitable to detect these (human) proteins in ELISA. However, we have the following antibodies available: 1) Beta-lactoglobulin: Unfortunately, I was unable to find a human ort...

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