Overview

  • Product name
    Human LAG3 ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    45 pg/ml
  • Range
    45 pg/well - 12000 pg/well
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 78.4 70% - 86%
    Plasma 78.2 68% - 95%
    Cell culture media 76.83 69% - 85%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s LAG3 (lymphocyte activation gene 3) Human ELISA Kit (ab193707) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human LAG3 in serum, plasma and cell culture supernatant. (UniprotID: P18627)


    This assay employs an antibody specific for human LAG3 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures LAG3 present in the samples. The wells are washed and biotinylated anti-human LAG3 is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of LAG3 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent D 1 x 15ml
    5X Assay Diluent B 1 x 15ml
    Biotinylated Human LAG3 detection antibody (lyophilized) 2 vials
    Human LAG3 Standards (lyophilized) 2 vials
    Pre-coated Human LAG3 Microplate (12 strips x 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Function
    Involved in lymphocyte activation. Binds to HLA class-II antigens.
  • Tissue specificity
    On cell surface of activated NK and T-lymphocytes.
  • Sequence similarities
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Alternative names
    • CD223
    • LAG-3
    • Lag3
    • LAG3_HUMAN
    • Lymphocyte activation gene 3 protein
    • Protein FDC
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab193707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Protocols

References

ab193707 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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