• Product name
    Human Laminin ELISA Kit
    See all Laminin kits
  • Detection method
  • Sample type
    Cell culture supernatant, Serum, Plasma, Heparin Plasma, EDTA Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    156 pg/ml - 10000 pg/ml
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Human Laminin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human Laminin in cell culture supernatants, serum and plasma (heparin, EDTA).

    A Laminin specific rabbit polyclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from rabbit specific for Laminin is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates ware washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human Laminin amount of sample captured in plate.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    ABC Diluent Buffer 1 x 12ml
    Antibody Diluent Buffer 1 x 12ml
    Anti-Human Laminin antibody Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti-Human Laminin antibody 1 x 130µl
    Lyophilized Human Laminin standard 2 x 10ng
    Plate Seal 1 x 4 units
    Sample Diluent Buffer 1 x 30ml
    TMB Color Developing Agent 1 x 10ml
    TMB Stop Solution 1 x 10ml
  • Research areas
  • Function
    Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • Involvement in disease
    Defects in LAMB2 are the cause of Pierson syndrome (PIERSS) [MIM:609049]; also known as microcoria-congenital nephrotic syndrome. Pierson syndrome is characterized by nephrotic syndrome with neonatal onset, diffuse mesangial sclerosis and eye abnormalities with microcoria as the leading clinical feature. Death usually occurs within the first weeks of life. Disease severity depends on the mutation type: nontruncating LAMB2 mutations may display variable phenotypes ranging from a milder variant of Pierson syndrome to isolated congenital nephrotic syndrome.
    Defects in LAMB2 are a cause of congenital nephrotic syndrome (CONPHS) [MIM:609049]. Congenital nephrotic syndrome constitutes a heterogeneous group of conditions having in common the disruption of normal glomerular permselectivity. Congenital nephrotic syndrome due to LAMB2 mutations may be associated with ocular abnormalities.
  • Sequence similarities
    Contains 13 laminin EGF-like domains.
    Contains 1 laminin IV type B domain.
    Contains 1 laminin N-terminal domain.
  • Domain
    The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
    Domains VI and IV are globular.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix > basement membrane. S-laminin is concentrated in the synaptic cleft of the neuromuscular junction.
  • Information by UniProt
  • Alternative names
    • Lamb2
    • LAMC1
    • LAMC2
    • LAMC3
    • laminin A
    • Laminin B
    • Laminin B1s chain
    • Laminin gamma 1
    • Laminin gamma 2
    • Laminin gamma 3
    • Laminin subunit beta-2
    • Laminin-11 subunit beta
    • Laminin-14 subunit beta
    • Laminin-15 subunit beta
    • Laminin-3 subunit beta
    • Laminin-4 subunit beta
    • Laminin-7 subunit beta
    • Laminin-9 subunit beta
    • OCCM
    • S-LAM beta
    • S-laminin subunit beta
    see all
  • Database links


Our Abpromise guarantee covers the use of ab119599 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Standard curve: mean of duplicates (+/-SD) with background readings subtracted

  • Hu laminin measured in various biological fluids showing quantity (pg) per mL of tested sample



ab119599 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Below is a link to our ELISA sample preparation guide, which should be of use to you:


For tissue we recommend:

Dissect the tissue of interest with clean tools, on ice p...

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The kit can recognize alpha, beta and gamma subunits of laminin.

Thank you for your enquiry and for your patience. It has taken some time to confirm this information for you.

I can confirm that the immunogen for the coating antibody in ab119599Laminin Human ELISA Kitisa complex of many subunits of Laminin...

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