Overview

  • Product name

    Human LEC ELISA Kit (CCL16)
    See all LEC kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 8 pg/ml
  • Range

    8.23 pg/ml - 6000 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 96.47 85% - 104%
    Serum 95.29 84% - 103%
    Plasma 94.68 83% - 103%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s LEC (CCL16) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human LEC in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human LEC coated on a 96-well plate. Standards and samples are pipetted into the wells and LEC present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human LEC antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of LEC bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    300X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human LEC 2 vials
    LEC Microplate (12 x 8 wells) 1 unit
    Recombinant Human LEC Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Function

    Shows chemotactic activity for lymphocytes and monocytes but not neutrophils. Also shows potent myelosuppressive activity, suppresses proliferation of myeloid progenitor cells. Recombinant SCYA16 shows chemotactic activity for monocytes and THP-1 monocytes, but not for resting lymphocytes and neutrophils. Induces a calcium flux in THP-1 cells that were desensitized by prior expression to RANTES.
  • Tissue specificity

    Mainly expressed in liver, also found in spleen and thymus. Highly expressed in LPS- and IFN-gamma-activated monocytes, weakly in some lymphocytes, including natural killer cells, gamma-delta T-cells, and some T-cell clones.
  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • C-C motif chemokine 16
    • CCL16
    • CCL16_HUMAN
    • Chemokine CC-4
    • Chemokine LEC
    • CKb12
    • HCC-4
    • HCC4
    • IL-10-inducible chemokine
    • IL10 inducible chemokine
    • ILINCK
    • LCC-1
    • LCC1
    • Liver CC chemokine 1 precursor
    • Liver expressed chemokine
    • Liver-expressed chemokine
    • LMC
    • Lymphocyte and monocyte chemoattractant
    • Monotactin 1
    • Monotactin-1
    • MTN-1
    • Mtn1
    • NCC-4
    • NCC4
    • New CC chemokine 4
    • SCYA16
    • SCYL4
    • Small inducible cytokine A16 precursor
    • Small inducible cytokine subfamily A (Cys Cys) member 16
    • Small-inducible cytokine A16
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100532

  • Representative standard curve using ab100532

Protocols

References

This product has been referenced in:

  • Hayatbakhsh MM  et al. The Serum Levels of CCL2 and CCL16 Expression in Patients with Irritable Bowel Syndrome. Rep Biochem Mol Biol 8:9-14 (2019). Read more (PubMed: 31334281) »
See 1 Publication for this product

Customer reviews and Q&As

LEC concentration in 16 human serum samples

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Human sera were diluted 10 times by Diluent A.

Abcam user community

Verified customer

Submitted Jun 04 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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