Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Product nameHuman Anti-Leishmania IgG ELISA Kit
Intra-assay Sample n Mean SD CV% Pos.Serum 7 7.4% Inter-assay Sample n Mean SD CV% Pos.Serum 4 5.9%
Sample typeSerum, Hep Plasma, Cit plasma
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s anti-Leishmania IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Leishmania in Human serum and plasma.
A 96-well plate has been precoated with Leishmania antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Leishmania-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Leishmania IgG sample captured in plate.
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover foil 1 unit IgG Sample Diluent Yellow, White cap 1 x 100ml Leishmania (IgG) Coated Microplate (12 x 8 wells) 12 strips of 8 1 unit Leishmania IgG Cut-off Control Yellow, Green cap 1 x 3ml Leishmania IgG Negative Control Yellow, Blue cap 1 x 2ml Leishmania IgG Positive Control Yellow, Red cap 1 x 2ml Leishmania Protein A Conjugate Blue, Black cap 1 x 20ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml
ab108749 has not yet been referenced specifically in any publications.