Overview

  • Product name

    Human Anti-Leishmania IgG ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Pos.Serum 7 7.4%
    Inter-assay
    Sample n Mean SD CV%
    Pos.Serum 4 5.9%
  • Sample type

    Serum, Hep Plasma, Cit plasma
  • Assay type

    Indirect
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s anti-Leishmania IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Leishmania in Human serum and plasma.

     

    A 96-well plate has been precoated with Leishmania antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Leishmania-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Leishmania IgG sample captured in plate.

  • Tested applications

    Suitable for: Indirect ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use at an assay dependent dilution.

Protocols

References

ab108749 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Q&A

Answer

This product has been tested with L.infantum in human serum or plasma collected with citrate. Other Leishmania species hasn’t been tested so we are unsure how well this kit will perform. In case you are interested in buying and trying this product then I would encourage you to participate in our 100% Abtrial discount programme - a free product can be claimed in your next order in exchange of review for this product. Please contact me with the order number for Abtrial code once the purchase is completed.

More details about the offer can be found at the following link;

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

This Abtrial offer was created to give scientists the opportunity to try products in an untested species or application, without the financial risk of failure.

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Question
Answer

Thank you for your enquiry.


Since Iraq is a sanctioned country by the USA, I am unable to provide you product information or sell you any products. All Abcam offices abide by these same restrictions.

Thank you for your understanding.

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