Overview

  • Product name

    Human Leptin ELISA Kit
    See all Leptin kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 2 pg/ml
  • Range

    1.64 pg/ml - 400 pg/ml
  • Recovery

    94 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.42 84% - 104%
    Serum 94.49 83% - 103%
    Plasma 93.85 82% - 102%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human Leptin ELISA kit is designed for the quantitative measurement of Human Leptin in serum, plasma, and cell culture supernatants.


    This assay employs an antibody specific for Human Leptin coated on a 96-well plate. Standards and samples are pipetted into the wells and Leptin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Leptin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get results in 90 minutes with Human Leptin ELISA Kit (ab179884) from our SimpleStep ELISA® range.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab100581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Leptin measured in cell lysates showing quantity (pg) per mg of protein

  • Leptin measured in biological fluids showing quantity (pg) per mL of tested sample

  • Representative Standard Curve using ab100581.

  • Representative Standard Curve using ab100581.

Protocols

References

This product has been referenced in:

  • Ashktorab H  et al. Adiponectin, Leptin, IGF-1, and Tumor Necrosis Factor Alpha As Potential Serum Biomarkers for Non-Invasive Diagnosis of Colorectal Adenoma in African Americans. Front Endocrinol (Lausanne) 9:77 (2018). ELISA . Read more (PubMed: 29593647) »
See 1 Publication for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Abreviews
We used rhesus macaque serum/plasma with the human leptin kit and used human serum as a positive control. The human serum produced a strong signal but virtually no signal was produced by the rhesus macaque serum/plasma. Therefore, it is likely that this kit does not cross react with non-human primate serum/plasma.

Mr. Joshua Preston

Verified customer

Submitted Mar 08 2019

Human Leptin ELISA test for rhesus macaques plasma

Excellent Excellent 5/5 (Ease of Use)
Abreviews
This ELISA kit is unsuitable for rhesus macaques. Undiluted plasma samples from 17 rhesus macaques yielded mostly negative or very weakly reactive positive results.

Abcam user community

Verified customer

Submitted Nov 02 2015

Question
Answer

These products are very similar, they use similar techniques to measure human Leptin in samples. In reviewing the information about each kit I have noted that ab100581 Sensitivity< 2 pg/ml, Range of 1.64 pg/ml - 400 pg/ml and Recovery of 94%. While ab108879 has a Sensitivity of 0.12 ng/ml, Range of 0.125 ng/ml - 32 ng/ml, and Recovery = 99.5%. So differences are mainly in sensitivity and recovery.

Read More

Answer

Thank you for contacting us. We do not specifically have data on adolescents but can tell you that typically human urine does not have to be concentrated and can be diluted using the kit if necessary.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for contacting us.

The differences of the two kits you are interested in are listed below. The most important difference is that one of the Kits is for detection of the Leptin Receptor, while the other kit is only detecting the secreted Leptin.

Leptin Human ELISA Kit (ab100581)

Price: €490.00
Order now and get it on Monday 12 November 2012
Sample type: Cell culture supernatant, Urine, Serum, Plasma
Sensitivity < 2 pg/ml
Range 1.64 pg/ml - 400 pg/ml
Function: Leptin is a secreted protein of 16 kDa and may function as part of a signalling pathway.



LeptinR Human ELISA Kit (ab119574)

Price: €475.00
The lead time on this item is currently 1-2 weeks
Sample type: Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
Sensitivity < 8 pg/ml
Range 156 pg/ml - 10000 pg/ml
Function: Receptor for obesity factor (leptin) 132 kDa.



As far as I am informed a proforma for payment was sent by the customer service team. The transport and packaging fees for Portugal are 60 Euro.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you very much for your inquiry and your interest in our kits.

Unfortunately,the cross-reactivity of ab108879and ab108881 with pig samples is low, around 5%.

For the other three kits, ab100581, ab100718 and ab100773,the laboratory has not tested these on pig samples and no prediction can be made, as not all epitopes are known.

For the most part we do not see cross-species reactivity with sandwich based assays. If you would however nevertheless like to test on of these kits, please do let me know, as I would be able to provide you with an interesting testing offer.

Please do also let me know whether you would like to discuss other options. Indeed, we have many individual antibodies against leptin in our catalog as well. Some of them are tested in pig (ab3583 and ab16227) and others are tested in ELISA. Again, we could discuss options and provide testing offers if you are interested in this.

The testing offers would be along the line of the offer described here:
https://www.abcam.com/collaborationdiscount

I hope this information is helpful. I am looking forward to hear back from you.

Read More

Answer

Thank you for contacting Abcam. Unfortunately, we do not have any cross reactivity data for rabbit samples on the ab100581 kit. Therefore we do not know if the kit will work with rabbit serum samples. Both the capture and detection antibody are monoclonal so unless the homology between human and rabbit is very high, I do not think that there would be any cross reactivity. Please let me know if there is anything else I can help with.  

Read More

Answer

Thank you for your enquriy. I am sorry to confirm that the Leptin standard protein from kit ab100581 is not sold separately. If you have any further questions, please do not hesitate to contact us.

Read More

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