Overview

  • Product name

    Human Leptin ELISA Kit
    See all Leptin kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 2 pg/ml
  • Range

    1.64 pg/ml - 400 pg/ml
  • Recovery

    94 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.42 84% - 104%
    Serum 94.49 83% - 103%
    Plasma 93.85 82% - 102%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human Leptin ELISA kit is designed for the quantitative measurement of Human Leptin in serum, plasma, and cell culture supernatants.


    This assay employs an antibody specific for Human Leptin coated on a 96-well plate. Standards and samples are pipetted into the wells and Leptin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Leptin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get results in 90 minutes with Human Leptin ELISA Kit (ab179884) from our SimpleStep ELISA® range.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab100581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Leptin measured in cell lysates showing quantity (pg) per mg of protein

  • Leptin measured in biological fluids showing quantity (pg) per mL of tested sample

  • Representative Standard Curve using ab100581.

  • Representative Standard Curve using ab100581.

Protocols

References

This product has been referenced in:

  • Ashktorab H  et al. Adiponectin, Leptin, IGF-1, and Tumor Necrosis Factor Alpha As Potential Serum Biomarkers for Non-Invasive Diagnosis of Colorectal Adenoma in African Americans. Front Endocrinol (Lausanne) 9:77 (2018). ELISA . Read more (PubMed: 29593647) »
See 1 Publication for this product

Customer reviews and Q&As

Filter by Ratings

1-2 of 2 Abreviews

Abreviews
We used rhesus macaque serum/plasma with the human leptin kit and used human serum as a positive control. The human serum produced a strong signal but virtually no signal was produced by the rhesus macaque serum/plasma. Therefore, it is likely that this kit does not cross react with non-human primate serum/plasma.

Mr. Joshua Preston

Verified customer

Submitted Mar 08 2019

Human Leptin ELISA test for rhesus macaques plasma

Excellent Excellent 5/5 (Ease of Use)
Abreviews
This ELISA kit is unsuitable for rhesus macaques. Undiluted plasma samples from 17 rhesus macaques yielded mostly negative or very weakly reactive positive results.

Abcam user community

Verified customer

Submitted Nov 02 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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