Overview

  • Product name

    Human Leptin ELISA Kit
    See all Leptin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    HeLa extract 5 2.4%
    Inter-assay
    Sample n Mean SD CV%
    HeLa extract 3 2.7%
  • Sample type

    Cell culture supernatant, Serum, Plasma, Hep Plasma, EDTA Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    2.3 pg/ml
  • Range

    11.7 pg/ml - 750 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 95 90% - 99%
    Cell culture media 116 114% - 119%
    Hep Plasma 116 108% - 122%
    EDTA Plasma 106 103% - 108%
    Cit plasma 109 104% - 111%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human Leptin SimpleStep ELISA® kit has been re-developed. We have identified new recombinant monoclonal antibodies to provide improved performance and consistency. This version will be discontinued February 2020 unless inventory is depleted beforehand.


    Leptin in vitro SimpleStep ELISA® kit is designed for the accurate quantitative measurement of Leptin protein in Human serum, plasma and cell culture supernatants.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    Leptin is a secreted protein factor produced by adipocytes. It regulates energy balance and body fat deposits. Leptin deficiency in humans and mice can cause in obesity.Acute low levels of leptin triggers increased appetite and chronic leptin deficiency can block the onset of puberty.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab179884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of Leptin were interpolated from the Leptin standard curve and corrected for sample dilution. The mean Leptin concentration was determined to be 6,260 pg/mL in plasma (citrate) and 1,845 pg/mL in serum.

  • Serum from 10 apparently healthy male donors was measured in triplicate for Leptin. The mean Leptin concentration was determined to be 2174 pg/mL with a range of 443 – 6250 pg/mL (Leptin was not detected in serum from Donor 7).

Protocols

References

This product has been referenced in:

  • Llobet L  et al. OXPHOS xenobiotics alter adipogenic differentiation at concentrations found in human blood. Dis Model Mech N/A:N/A (2015). Functional Studies ; Human . Read more (PubMed: 26398948) »
See 1 Publication for this product

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