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|Sample type||Average %||Range|
|Cell culture supernatant||97.41||84% - 103%|
|Serum||94.26||83% - 106%|
|Plasma||93.64||82% - 102%|
Abcam’s LIF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human LIF in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human LIF coated on a 96-well plate. Standards and samples are pipetted into the wells and LIF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human LIF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of LIF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
LIF (Leukocyte inhibitory factor) is produced by various fibroblast cell lines, antigen-stimulated alloreactive T-lymphocytes, mitogen-activated spleen cells, and Krebs and Ehrlich ascites cells. It is also produced by monocytes after cell activation. This factor inhibits the random and directed migration of polymorphonuclear (PMN) leukocytes. LIF also induces specific granule secretion by PMNs and potentiates many responses mediated by the chemotactic compound fMLP (formyl-methionyl-leucylphenylalanine).
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|320X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent B||1 x 15ml|
|Assay Diluent A||1 x 30ml|
|Biotinylated anti-Human LIF||2 vials|
|LIF Microplate (12 strips x 8 wells)||1 unit|
|Recombinant Human LIF Standard (lyophilized)||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100582 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab100582 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"