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|Sample type||Average %||Range|
|Cell culture supernatant||96.25||86% - 105%|
|Plasma||97.63||85% - 104%|
Abcam’s LIGHT Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human LIGHT in plasma, and cell culture supernatants. (Human LIGHT concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human LIGHT coated on a 96-well plate. Standards and samples are pipetted into the wells and LIGHT present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human LIGHT antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of LIGHT bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Human LIGHT in normal serum with ab100583, only in serum spiked with Human LIGHT.
Optimization may be required with urine samples.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|300X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent B||1 x 15ml|
|Assay Diluent A||1 x 30ml|
|Biotinylated anti-Human LIGHT||2 vials|
|LIGHT Microplate (12 x 8 wells)||1 unit|
|Recombinant Human LIGHT Standard (lyophilized)||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100583 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab100583 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"