Key features and details
- Sensitivity: 10 pg/ml
- Range: 156 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman Lipocalin-2 ELISA Kit (NGAL)
See all Lipocalin-2 / NGAL kits
Intra-assay Sample n Mean SD CV% 1 16 256pg/ml 19.2 = 7.5% 2 16 1510pg/ml 81.54 = 5.4% 3 16 5422pg/ml 281.94 = 5.2% Inter-assay Sample n Mean SD CV% 1 24 254pg/ml 19.05 = 7.5% 2 24 1537pg/ml 84.53 = 5.5% 3 24 5138pg/ml 323.69 = 6.3%
Sample typeCell culture supernatant, Saliva, Urine, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 10 pg/ml
Range156 pg/ml - 10000 pg/ml
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s Human Lipocalin-2 (NGAL) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human Lipocalin-2 in cell culture supernatants, serum, plasma (heparin), saliva and urine.
A Lipocalin-2 specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for Lipocalin-2 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human Lipocalin-2 amount of sample captured in plate.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human Lipcalin-2 antibody Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl Biotinylated anti-Human Lipocalin-2 antibody 1 x 130µl Lyophilized recombinant Human Lipocalin-2 standard 2 x 10ng Plate Seal 1 x 4 units Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml
FunctionIron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
Tissue specificityExpressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes.
Sequence similaritiesBelongs to the calycin superfamily. Lipocalin family.
Cellular localizationSecreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.
- Information by UniProt
- 25 kDa alpha-2-microglobulin related subunit of MMP-9
- 25 kDa alpha-2-microglobulin-related subunit of MMP-9
ab119600 has been referenced in 3 publications.
- Jia HM et al. Derivation and validation of plasma endostatin for predicting renal recovery from acute kidney injury: a prospective validation study. Crit Care 22:305 (2018). PubMed: 30445971
- Wu J et al. Urinary RBP and NGAL Levels are Associated with Nephropathy in Patients with Type 2 Diabetes. Cell Physiol Biochem 42:594-602 (2017). PubMed: 28954270
- Guiddir T et al. Lipocalin 2 in cerebrospinal fluid as a marker of acute bacterial meningitis. BMC Infect Dis 14:276 (2014). WB . PubMed: 24885531