Overview

  • Product name

    Human Lipoprotein A ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4.6%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9.5%
  • Sample type

    Cell culture supernatant, Milk, Urine, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    = 0.7 ng/ml
  • Range

    0.78 ng/ml - 50 ng/ml
  • Recovery

    = 98 %

  • Assay time

    4h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Lipoprotein A Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Lipoprotein A concentrations in plasma, serum, urine, milk, cerebrospinal fluid (CSF) and cell culture supernatants.


    A Lipoprotein A specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Lipoprotein A specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Lipoprotein A captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent M Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Human Lipoprotein A Antibody 1 x 120µl
    Chromogen Substrate 1 x 8ml
    Lipoprotein A Microplate (12 x 8 well strips) 1 unit
    Lipoprotein A Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas

  • Function

    Apo(a) is the main constituent of lipoprotein(a) (Lp(a)). It has serine proteinase activity and is able of autoproteolysis. Inhibits tissue-type plasminogen activator 1. Lp(a) may be a ligand for megalin/Gp 330.
  • Sequence similarities

    Belongs to the peptidase S1 family. Plasminogen subfamily.
    Contains 38 kringle domains.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications

    N- and O-glycosylated. The N-glycans are complex biantennary structures present in either a mono- or disialylated state. The O-glycans are mostly (80%) represented by the monosialylated core type I structure, NeuNAcalpha2-3Galbeta1-3GalNAc, with smaller amounts of disialylated and non-sialylated O-glycans also detected.
  • Information by UniProt
  • Alternative names

    • Apo(a)
    • APOA_HUMAN
    • Apolipoprotein(a)
    • Lp(a)
    • LPA
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108878 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Images

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted
  • Lipoprotein A measured in biological fluids and cell culture supernatants showing quantity (ng) per mL of tested sample. Human serum, plasma and milk samples diluted 4000-32000 fold. Human urine and saliva diluted 2-8 fold. Mouse and rat samples diluted 10-100 fold. Cell supernatants diluted 1-10 fold.

Protocols

References

This product has been referenced in:

  • Liu B  et al. Danshen (Salvia miltiorrhiza) Compounds Improve the Biochemical Indices of the Patients with Coronary Heart Disease. Evid Based Complement Alternat Med 2016:9781715 (2016). Read more (PubMed: 27366196) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer



I can confirm that the antibody used in the kit are polyclonal. I am sorry that the immunogen is confidential in this case.

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Answer

Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is having problems with this product. We have tested ab108878 batch: GR57178 and it has passed QC. Though you have kindly provided some details, it would be much appreciated if I could get some more information which would help me identify the source of the problem. - How did the customer prepare the std points and the samples? - How did she dilute biotin? - How did she dilute SP? Thank you for your understanding and co-operation in this matter. I look forward to hearing from you and hope to solve this problem as soon as possible.

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Answer

Thank you for contacting us. The information regarding the sample and standard dilutions is included under Point 6 of the Protocol “Preparation of Reagents” (Page 7). As you mention, for plasma samples, the recommended dilution is 1/8000. The table on page 14 refers to the linearity obtained for different sample types at different dilutions. The best % results for Plasma are those obtained at dilutions 1/8000 (101% of expected value). When using Serum sample, the most accurate value is obtained when diluting the samples 1/8000, for Milk the optimal dilution is 1/4000, whereas for Urine, 100% of the expected value is obtained when diluting 1/4. In the case you are still experiencing problems with any of the other kits, please do let us know, and we will be happy to help you. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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