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Dear technical team, Our customer purchased 3 of ab108878 and has some questions. 1. Her sample is plasma and following protocol page 14, she diluted her plasma sample as 1:8000. I don't know this table means dilution factor for each samples so, please let me know if it is right. 2. She used one kit and applied standard and samples but, any of well changed blue after adding substrate even standard. She is going to try another one of kit and needs sample dilution information. Check this please Best regards,
Asked on Oct 05 2011
Thank you for contacting us. The information regarding the sample and standard dilutions is included under Point 6 of the Protocol “Preparation of Reagents” (Page 7). As you mention, for plasma samples, the recommended dilution is 1/8000. The table on page 14 refers to the linearity obtained for different sample types at different dilutions. The best % results for Plasma are those obtained at dilutions 1/8000 (101% of expected value). When using Serum sample, the most accurate value is obtained when diluting the samples 1/8000, for Milk the optimal dilution is 1/4000, whereas for Urine, 100% of the expected value is obtained when diluting 1/4. In the case you are still experiencing problems with any of the other kits, please do let us know, and we will be happy to help you. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Oct 05 2011