Question (25159) | Human Lipoprotein A ELISA Kit (ab108878)

LOT NUMBER GR57178 ORDER NUMBER 950837 DESCRIPTION OF THE PROBLEM No signal or weak signal SAMPLE human Plasma PRIMARY ANTIBODY coated plated, wash : followed the kit protocol DETECTION METHOD added 50ul streptavidin peroxidase conjugate the each well incubation 30min POSITIVE AND NEGATIVE CONTROLS USED standard(25, 12.5, 6.25,3.13, 1.56, 0.78) ANTIBODY STORAGE CONDITIONS 4C TYPE OF ELISA sandwich ELISA COATING WELL pre coated incubation: 2hour (after adding sample and standard) BLOCKING CONDITIONS no SECONDARY ANTIBODY Biotinylated hman Lipoprotein anatibody 50ul each well wash : followed the kit protocol time : one hour HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 2 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? no. our customer followed the kit's protocol ADDITIONAL NOTES She could not see any results in sample and standard. There was no color change. (as you know the plate had to be changed as blue in sample and standard) she bought 3kit and used 2kis, the 2kit had sample problem.