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LOT NUMBER GR57178 ORDER NUMBER 950837 DESCRIPTION OF THE PROBLEM No signal or weak signal SAMPLE human Plasma PRIMARY ANTIBODY coated plated, wash : followed the kit protocol DETECTION METHOD added 50ul streptavidin peroxidase conjugate the each well incubation 30min POSITIVE AND NEGATIVE CONTROLS USED standard(25, 12.5, 6.25,3.13, 1.56, 0.78) ANTIBODY STORAGE CONDITIONS 4C TYPE OF ELISA sandwich ELISA COATING WELL pre coated incubation: 2hour (after adding sample and standard) BLOCKING CONDITIONS no SECONDARY ANTIBODY Biotinylated hman Lipoprotein anatibody 50ul each well wash : followed the kit protocol time : one hour HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 2 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? no. our customer followed the kit's protocol ADDITIONAL NOTES She could not see any results in sample and standard. There was no color change. (as you know the plate had to be changed as blue in sample and standard) she bought 3kit and used 2kis, the 2kit had sample problem.
Asked on Oct 07 2011
Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is having problems with this product. We have tested ab108878 batch: GR57178 and it has passed QC. Though you have kindly provided some details, it would be much appreciated if I could get some more information which would help me identify the source of the problem. - How did the customer prepare the std points and the samples? - How did she dilute biotin? - How did she dilute SP? Thank you for your understanding and co-operation in this matter. I look forward to hearing from you and hope to solve this problem as soon as possible.
Answered on Oct 07 2011