Overview

  • Product name

    Human Liver-FABP ELISA Kit
    See all liver FABP kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5 6.2%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 4.2%
  • Sample type

    Cell culture supernatant, Urine, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    9.4 pg/ml
  • Range

    14.8 pg/ml - 950 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 103 97% - 113%
    Urine 104 101% - 105%
    Serum 97 93% - 101%
    Heparin Plasma 85 81% - 92%
    EDTA Plasma 93 87% - 98%
    Citrate Plasma 88 82% - 94%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat, Cow
  • Product overview

    Liver-FABP in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of human Liver-FABP in serum, plasma, cell culture supernatant and urine.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    Liver-FABP is a 14 kDa member of the FABP family. Liver-FABP binds and transports cholesterol, free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules. A variety of cells express Liver-FABP, including hepatocytes, simple columnar epithelium of the small intestine, and proximal tubular epithelium of the kidney.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human Liver-FABP Capture Antibody 1 x 600µl
    10X Human Liver-FABP Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 5BI 1 x 6ml
    Human L-FABP Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport.
  • Sequence similarities

    Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • Domain

    Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Alternative names

    • FABP 1
    • FABP1
    • FABPL
    • FABPL_HUMAN
    • Fatty Acid Binding Protein
    • Fatty Acid Binding Protein 1
    • Fatty acid binding protein 1 liver
    • Fatty acid-binding protein
    • Fatty acid-binding protein 1
    • Fatty acid-binding protein liver
    • L FABP
    • L-FABP
    • liver
    • Liver-type fatty acid-binding protein
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab218261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of Liver-FABP were measured in duplicates, interpolated from the Liver-FABP standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 7.5%, plasma (citrate) 7.5%, plasma (heparin) 7.5%, plasma EDTA) 7.5%, urine 50 %, and spleen HepG2 supernatant 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Liver-FABP concentration was determined to be 11.2 ng/mL in serum, 12.2 ng/mL in plasma (citrate) and 13.6 ng/mL in plasma (heparin), 11.0 ng/mL in plasma (EDTA), 0.682 ng/mL in urine, and 11.4 ng/mL in HepG2 supernatant.

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Liver-FABP concentration was determined to be 10.3 ng/mL with a range of 6.36 – 19.0 ng/mL.

Protocols

References

ab218261 has not yet been referenced specifically in any publications.

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