Overview

  • Product name

    Human LOXL2 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 1589pg/ml 79.45 5%
    2 16 3122pg/ml 171.71 5.5%
    3 16 7510pg/ml 435.58 5.8%
    Inter-assay
    Sample n Mean SD CV%
    1 24 1466pg/ml 76.23 5.2%
    2 24 4236pg/ml 258.39 6.1%
    3 24 6998pg/ml 447.87 6.4%
  • Sample type

    Cell culture supernatant, Serum, Cell Lysate, Heparin Plasma, EDTA Plasma, Tissue Homogenate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    156 pg/ml - 10000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human LOXL2 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213808) is designed for the quantitative detection of Human LOXL2 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for LOXL2 has been pre-coated onto 96-well plates. Standards (NSO, Q26-Q774) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LOXL2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human LOXL2 amount of sample captured in plate.

  • Notes

    Lysyl oxidase homolog 2 is an enzyme that in humans is encoded by the LOXL2 gene. This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue; encoding an extracellular copper-dependent amine oxidase that catalyzes the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control and chemotaxis to each member of the family. LOXL2 can also crosslink collagen type IV and hence influence the sprouting of new blood vessels.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human LOXL2 coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti-Human LOXL2 antibody 1 x 130µl
    Lyophilized recombinant Human LOXL2 standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Tissue specificity

    Expressed in many tissues. Highest expression in reproductive tissues, placenta, uterus and prostate.
  • Sequence similarities

    Belongs to the lysyl oxidase family.
    Contains 4 SRCR domains.
  • Post-translational
    modifications

    The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine.
  • Cellular localization

    Secreted > extracellular space.
  • Information by UniProt
  • Alternative names

    • LOR 2
    • LOR2
    • LOX L2
    • LOXL 2
    • LOXL2
    • LOXL2_HUMAN
    • Lysyl oxidase homolog 2
    • Lysyl oxidase like 2
    • Lysyl oxidase like protein 2
    • Lysyl oxidase related 2
    • Lysyl oxidase related protein 2
    • Lysyl oxidase related protein WS9 14
    • Lysyl oxidase-like protein 2
    • Lysyl oxidase-related protein 2
    • Lysyl oxidase-related protein WS9-14
    • WS9 14
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human LOXL2 ELISA Kit (ab213808) Standard Curve

Protocols

References

ab213808 has not yet been referenced specifically in any publications.

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