Overview

  • Product name

    Human Luteinizing Hormone ELISA Kit
    See all Luteinizing Hormone kits
  • Detection method

    Colorimetric
  • Sample type

    Serum
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    = 1 mIU/ml
  • Range

    1 mIU/ml - 200 mIU/ml
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    ab108651 Luteinizing Hormone Human ELISA Kit is intended for the quantitative determination of luteinizing hormone (LH) concentration in human serum.

    Luteinizing hormone (LH) is produced in both men and women from the anterior pituitary gland in response to luteinizing hormone-releasing hormone (LH-RH or Gn-RH), which is released by the hypothalamus. LH, also called interstitial cell-stimulating hormone (ICSH) in men, is a glycoprotein with a molecular weight of approximately 30,000 daltons. It is composed of two noncovalently associated dissimilar amino acid chains, alpha and beta. The alpha chain is similar to that found in human thyroid-stimulating hormone (TSH), follicle-stimulating hormone (FSH), and human chorionic gonadotropin (hCG). The differences between these hormones lie in the amino acid composition of their beta subunits, which account for their immunological differentiation.

    The basal secretion of LH in men is episodic and has the primary function of stimulating the interstitial cells (Leydig cells) to produce testosterone. The variation in LH concentrations in women is subject to the complex ovulatory cycle of healthy menstruating women, and depends on a sequence of hormonal events along the gonado-hypothalamic-pituitary axis.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    Enzyme Conjugate Reagent 1 x 13ml
    LH Reference Standard 0 (0 mIU/ml) 1 unit
    LH Reference Standard 1 (5 mIU/ml) 1 unit
    LH Reference Standard 2 (15 mIU/ml) 1 unit
    LH Reference Standard 3 (50 mIU/ml) 1 unit
    LH Reference Standard 4 (100 mIU/ml) 1 unit
    LH Reference Standard 5 (200 mIU/ml) 1 unit
    Mouse monoclonal anti-α LH coated microtiter plate 1 unit
    Stop Solution 1 x 11ml
    TMB Reagent 1 x 11ml
  • Research areas

  • Function

    Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
  • Tissue specificity

    Pituitary gland.
  • Involvement in disease

    Defects in LHB are a cause of hypogonadism LHB-related (HGON-LHB) [MIM:152780]. Hypogonadism is characterized by infertility and pseudohermaphroditism.
  • Sequence similarities

    Belongs to the glycoprotein hormones subunit beta family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • CGB 4
    • CGB4
    • Interstitial cell stimulating hormone beta chain
    • LH B
    • LH-B
    • LHB
    • LSH B
    • LSH beta
    • LSH-B
    • LSH-beta
    • LSHB
    • LSHB_HUMAN
    • Luteinizing hormone beta
    • Luteinizing hormone beta polypeptide
    • Luteinizing hormone beta subunit
    • Luteinizing hormone subunit beta
    • Lutropin beta chain
    • Lutropin beta chain precursor
    • Lutropin subunit beta
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Images

Protocols

References

This product has been referenced in:

  • Saad DY  et al. Protective effects of sea cucumber (Holothuria atra) extract on testicular dysfunction induced by immune suppressant drugs in Wistar rats. Andrologia N/A:e13017 (2018). Read more (PubMed: 29687464) »
  • Zheng R  et al. A novel PNPLA6 compound heterozygous mutation identified in a Chinese patient with Boucher-Neuhäuser syndrome. Mol Med Rep 18:261-267 (2018). Read more (PubMed: 29749493) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer

Thank you very much for your inquiry.

We do have no ELISA kit against LH, FSH or Progesterone which are tested against the rat proteins. We do have however such kits tested against the human protein, for which we have however not yet tested whether they can work with the rat protein.

I am inquiring with the laboratory whether we have any anti rat ELISA kits for these proteins which we could put in ourcatalogue and I will get back to you as soon as I know more.

Thank you for your patience. Please let me know if you have any further questions.

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