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Abcam’s Luteinizing hormone (LH) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of LH in serum.
A 96-well plate has been precoated with Streptavidin. Samples, standards and the LH-HRP and Bitoin conjugate are added to the wells. Biotinylated monoclonal and enzyme labeled antibodies are added and the reactants mixed: these antibodies have high affinity and specificity and are directed against distinct and different epitopes of LH. Reaction between the various LH antibodies and native LH occurs in the microwells without competition or steric hindrance forming a soluble sandwich complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of LH in the sample and the intensity is measured at 450 nm.
|Components||1 x 96 tests|
|50X Washing Solution||1 x 20ml|
|Cover Foil||1 unit|
|Luteinizing Hormone Coated Microplate (12 x 8 wells)||1 unit|
|Luteinizing Hormone Control||1 x 1ml|
|Luteinizing Hormone HRP and Biotin Conjugate||1 x 12ml|
|Luteinizing Hormone Standard 0 - 0 mU/mL||1 x 1ml|
|Luteinizing Hormone Standard 1 - 5 mU/mL||1 x 1ml|
|Luteinizing Hormone Standard 2 - 25 mU/mL||1 x 1ml|
|Luteinizing Hormone Standard 3 - 50 mU/mL||1 x 1ml|
|Luteinizing Hormone Standard 4 - 100 mU/mL||1 x 1ml|
|Luteinizing Hormone Standard 5 - 200 mU/mL||1 x 1ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab178658 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"