Overview

  • Product name
    Human Luteinizing hormone ELISA Kit (LH)
    See all Luteinizing Hormone kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Serum 20 <=9.21%
    Inter-assay
    Sample n Mean SD CV%
    Serum 15 <=7.91%
  • Sample type
    Serum, Heparin Plasma, Citrate Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    0.22 mIU/ml
  • Range
    0.5 mU/ml - 200 mU/ml
  • Assay duration
    Multiple steps standard assay
  • Product overview

    Abcam’s Luteinizing hormone (LH) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of LH in serum.


    A 96-well plate has been precoated with Streptavidin. Samples, standards and the LH-HRP and Bitoin conjugate are added to the wells. Biotinylated monoclonal and enzyme labeled antibodies are added and the reactants mixed: these antibodies have high affinity and specificity and are directed against distinct and different epitopes of LH. Reaction between the various LH antibodies and native LH occurs in the microwells without competition or steric hindrance forming a soluble sandwich complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of LH in the sample and the intensity is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab178658 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab178658

Protocols

References

This product has been referenced in:
  • Dorostghoal M  et al. Overexpression of Endometrial Estrogen Receptor-Alpha in The Window of Implantation in Women with Unexplained Infertility. Int J Fertil Steril 12:37-42 (2018). Read more (PubMed: 29334205) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab178658.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up