Overview

  • Product name

    Human LYVE1 ELISA Kit
    See all LYVE1 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 3.4%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 8%
  • Sample type

    Urine, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    4 pg/ml
  • Range

    23.44 pg/ml - 1500 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine 105 101% - 108%
    Serum 108 103% - 115%
    Heparin Plasma 110 106% - 115%
    EDTA Plasma 110 105% - 117%
    Citrate Plasma 113 107% - 117%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    LYVE1 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of LYVE1 protein in human serum, plasmas, and urine.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Lymphatic Vessel Endothelial Hyaluronic Acid Receptor 1 (LYVE1), also known as Cell surface retention sequence-binding protein 1, Extracellular link domain-containing protein 1, and hyaluronic acid receptor is a CD44 homolog protein encoded by the LYVE1 gene. LYVE1 is a type I integral membrane glycoprotein that acts as a receptor to both soluble and immobilized hyaluronan and is located primarily on lymphatic endothelial cells. LYVE1 forms a disulfide- linked homodimer and interacts with PDGFB and IGFBP3.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human LYVE1 Capture Antibody 1 x 600µl
    10X Human LYVE1 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 4BR 1 x 6ml
    Human LYVE1 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.
  • Tissue specificity

    Mainly expressed in endothelial cells lining lymphatic vessels.
  • Sequence similarities

    Contains 1 Link domain.
  • Post-translational
    modifications

    O-glycosylated.
  • Cellular localization

    Membrane. Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand-dependent internalization and recycling at the cell surface.
  • Information by UniProt
  • Alternative names

    • Cell surface retention sequence-binding protein 1
    • CRSBP 1
    • CRSBP-1
    • CRSBP1
    • extracellular link domain containing 1
    • extracellular link domain-containing 1
    • Extracellular link domain-containing protein 1
    • HAR
    • Hyaluronic acid receptor
    • Lymphatic endothelium specific hyaluronan receptor
    • lymphatic vessel endothelial hyaluronan receptor 1
    • Lymphatic vessel endothelial hyaluronic acid receptor 1
    • LYVE 1
    • LYVE-1
    • LYVE1
    • LYVE1_HUMAN
    • XLKD1
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab238263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • The LYVE1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
  • The concentrations of LYVE1 were measured in duplicates, interpolated from the LYVE1 standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (EDTA) 1:500, plasma (citrate) 1:500, and plasma (heparin) 1:500. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LYVE1 concentration was determined to be 375 ng/mL in plasma (EDTA), 591 ng/mL in plasma (citrate) and 372 ng/mL in plasma (Heparin).
  • The concentrations of LYVE1 were measured in duplicates, interpolated from the LYVE1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:500, urine 1:20. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LYVE1 concentration was determined to be 486 ng/mL in serum and 33 ng/mL in urine.

Protocols

References

ab238263 has not yet been referenced specifically in any publications.

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