Product nameHuman Macrophage Stimulating Protein ELISA Kit
See all Macrophage Stimulating Protein kits
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 8 pg/ml
Range8.23 pg/ml - 2000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 97.25 86% - 106% Serum 94.48 83% - 104% Plasma 95.29 82% - 105%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s Macrophage Stimulating Protein Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Macrophage Stimulating Protein in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human Macrophage Stimulating Protein coated on a 96-well plate. Standards and samples are pipetted into the wells and Macrophage Stimulating Protein present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Macrophage Stimulating Protein antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Macrophage Stimulating Protein bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
Tested applicationsSuitable for: Sandwich ELISAmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human Macrophage Stimulating Protein 2 vials Macrophage Stimulating Protein Microplate (12 x 8 wells) 1 unit Recombinant Human Macrophage Stimulating Protein Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
RelevanceMacrophage Stimulating Protein contains four kringle domains and a serine protease domain, similar to that found in hepatic growth factor. Despite the presence of the serine protease domain, the encoded protein may not have any proteolytic activity. The receptor for this protein is RON tyrosine kinase, which upon activation stimulates ciliary motility of ciliated epithelial lung cells. This protein is secreted and cleaved to form an alpha chain and a beta chain bridged by disulfide bonds.
Our Abpromise guarantee covers the use of ab100612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
This product has been referenced in:
- Yu K et al. A multi-stage association study of plasma cytokines identifies osteopontin as a biomarker for acute coronary syndrome risk and severity. Sci Rep 9:5121 (2019). Read more (PubMed: 30914768) »
- Yang B et al. Exposure to Polycyclic Aromatic Hydrocarbons, Plasma Cytokines, and Heart Rate Variability. Sci Rep 6:19272 (2016). Read more (PubMed: 26758679) »