Overview

  • Product name

    Human Macrophage Stimulating Protein ELISA Kit
    See all Macrophage Stimulating Protein kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 8 pg/ml
  • Range

    8.23 pg/ml - 2000 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 97.25 86% - 106%
    Serum 94.48 83% - 104%
    Plasma 95.29 82% - 105%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Macrophage Stimulating Protein Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Macrophage Stimulating Protein in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human Macrophage Stimulating Protein coated on a 96-well plate. Standards and samples are pipetted into the wells and Macrophage Stimulating Protein present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Macrophage Stimulating Protein antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Macrophage Stimulating Protein bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human Macrophage Stimulating Protein 2 vials
    Macrophage Stimulating Protein Microplate (12 x 8 wells) 1 unit
    Recombinant Human Macrophage Stimulating Protein Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Relevance

    Macrophage Stimulating Protein contains four kringle domains and a serine protease domain, similar to that found in hepatic growth factor. Despite the presence of the serine protease domain, the encoded protein may not have any proteolytic activity. The receptor for this protein is RON tyrosine kinase, which upon activation stimulates ciliary motility of ciliated epithelial lung cells. This protein is secreted and cleaved to form an alpha chain and a beta chain bridged by disulfide bonds.
  • Alternative names

    • D3F15S2
    • D3F15S2h
    • D9H3F15S2
    • DNF15S2
    • DNF15S2h
    • Hepatocyte growth factor like protein
    • Hepatocyte growth factor like protein homolog
    • HGFL
    • Macrophage stimulating 1
    • Macrophage stimulatory protein
    • MSP
    • MST1
    • NF15S2
    see all

Applications

Our Abpromise guarantee covers the use of ab100612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab100612.

  • Representative Standard Curve using ab100612.

Protocols

References

This product has been referenced in:

  • Yu K  et al. A multi-stage association study of plasma cytokines identifies osteopontin as a biomarker for acute coronary syndrome risk and severity. Sci Rep 9:5121 (2019). Read more (PubMed: 30914768) »
  • Yang B  et al. Exposure to Polycyclic Aromatic Hydrocarbons, Plasma Cytokines, and Heart Rate Variability. Sci Rep 6:19272 (2016). Read more (PubMed: 26758679) »
See all 2 Publications for this product

Customer reviews and Q&As

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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