Human MAPK Phosphorylation Antibody Array (Membrane, 17 Targets) (ab211061)

Overview

  • Product name
    Human MAPK Phosphorylation Antibody Array (Membrane, 17 Targets)
  • Sample type
    Cell Lysate, Tissue Lysate
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Human MAPK Phosphorylation Antibody Array (ab211061) for use with cell and tissue lysates.


    Targets: Akt (pS473), CREB (pS133), ERK1 (pT202/Y204)/ERK2 (pT185/Y187), GSK3a (pS21), GSK3b (pS9), HSP27 (pS82), JNK (pT183), MEK (pS217/221), MKK3 (pS189), MKK6 (pS207), MSK2 (pS360), mTOR (pS2448), p38 (pT180/Y182), p53 (pS15), P70S6K (pT421/S424), RSK1 (pS380), RSK2 (pS386).


     

  • Notes

    Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1 mL of 1 sample to each membrane), and then paired detector antibodies and HRP-Anti-Rabbit IgG. The antibody array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.

    Learn more about cytokine arrays and other membrane antibody arrays

  • Tested applications
    Suitable for: Multiplex Protein Detectionmore details

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 2 Membrane 4 Membrane 8 Membrane
    1,000X HRP-Anti-Rabbit IgG 1 x 20µl 1 x 20µl 1 x 20µl
    100X Phosphatase Inhibitor Cocktail Set I Concentrate 1 vial 1 vial 2 vials
    20X Wash Buffer I 1 x 10ml 1 x 10ml 1 x 20ml
    20X Wash Buffer II 1 x 10ml 1 x 10ml 1 x 20ml
    2X Cell Lysis Buffer 1 x 10ml 1 x 10ml 1 x 16ml
    8-Well Incubation Tray (with Lid) 1 unit 1 unit 1 unit
    Antibody Arrays 2 units 4 units 8 units
    Detection Antibody Cocktail 1 vial 2 vials 4 vials
    1X Blocking Buffer 1 x 25ml 1 x 25ml 2 x 25ml
    Detection Buffer C 1 x 1.5ml 1 x 1.5ml 1 x 2.5ml
    Detection Buffer D 1 x 1.5ml 1 x 1.5ml 1 x 2.5ml
    Phosphatase Inhibitor Cocktail Set II 1 vial 1 vial 2 vials
    Plastic sheets 1 unit 1 unit 1 unit
    Protease Inhibitor Cocktail 1 vial 1 vial 2 vials

Applications

Our Abpromise guarantee covers the use of ab211061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Multiplex Protein Detection Use at an assay dependent concentration.

Images

  • HeLa cells were grown to 80% confluency and then serum starved overnight. Cells were either untreated (bottom panel) or treated (top panel) with 250 nM PMA for 20 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system.

  • HeLa cells were grown to 80% confluency and then serum starved overnight. Cells were either untreated or treated with 250 nM PMA for 20 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system.

  • HepG2 cells were grown to 80% confluency and then serum starved overnight.  Cells were either untreated (bottom panel) or treated (top panel) with 25 ng/mL of recombinant human IL-1β for 30 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system.

  • HepG2 cells were grown to 80% confluency and then serum starved overnight.  Cells were either untreated or treated  with 25 ng/mL of recombinant human IL-1β for 30 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system.  

Protocols

References

ab211061 has not yet been referenced specifically in any publications.

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