Overview

  • Product name

    Human MASPIN ELISA Kit
    See all MASPIN kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 8 3.8%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 1.4%
  • Sample type

    Cell culture extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    8 pg/ml
  • Range

    0.11 ng/ml - 7 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 108 107% - 109%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    MASPIN in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of MASPIN protein in human cell extracts.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    MASPIN (Mammary Serine Protease Inhibitor) is a 375 amino acid glycosylated protein encoded by the gene SERPINB5. It belongs to a family of proteins known as serpins (serine protease inhibitor), which function as regulators of protein breakdown by acting as suicide inhibitors of the catalytic activity of proteinases. Inhibitory serpins undergo a transition between a stressed and relaxed confirmation, where the stressed confirmation binds to the catalytic serine residue of the protease. MASPIN is thought to interact with extracellular matrix components, glutathione S-transferase, and interferon regulatory factor 6. It was originally thought to play a role in tumor metastasis, but the exact function is unknown.   

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human MASPIN Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 4BI 1 x 6ml
    Human MASPIN Capture Antibody (Lyophilized) 1 vial
    Human MASPIN Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
  • Tissue specificity

    Normal mammary epithelial cells.
  • Sequence similarities

    Belongs to the serpin family. Ov-serpin subfamily.
  • Cellular localization

    Secreted > extracellular space.
  • Information by UniProt
  • Alternative names

    • Maspin
    • Ovalbumin
    • Peptidase inhibitor 5
    • PI-5
    • PI5
    • Protease inhibitor 5
    • protease inhibitor 5 (maspin)
    • Serine (or cysteine) peptidase inhibitor, clade B, member 5
    • serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5
    • Serpin B5
    • serpin family B member 5
    • Serpin peptidase inhibitor
    • Serpin peptidase inhibitor, clade B (ovalbumin), member 5
    • Serpinb5
    • SPB5_HUMAN
    • Spi5
    • Spi7
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab218269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of MASPIN were measured in duplicate, interpolated from the MASPIN standard curve, and corrected for sample dilution. The interpolated, dilution factor-corrected values are plotted (mean +/- SD, n=2). The mean MASPIN concentration was determined to be 57.5 ng/mL in PC-3 cell extract.

  • The concentrations of MASPIN were measured in duplicate, interpolated from the MASPIN standard curve. The interpolated, dilution factor-corrected values are plotted (mean +/- SD, n=2). The mean MASPIN concentration was determined to be 55 ng/mL, 93 ng/mL, and non-detectable in PC-3, A431, HL-60, and HeLa cell extract, respectively.

Protocols

References

This product has been referenced in:

  • Nacif-Pimenta R  et al. Differential responses of epithelial cells from urinary and biliary tract to eggs of Schistosoma haematobium and S. mansoni. Sci Rep 9:10731 (2019). Read more (PubMed: 31341177) »
See 1 Publication for this product

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