Overview

  • Product name

    Human MASPIN Matched Antibody Pair Kit
    See all MASPIN kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Range

    62.5 pg/ml - 4000 pg/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant Human MASPIN.


    Matched antibody pair kits and reagents deliver consistent, specific, and sensitive results.



    • Batch-to-batch consistency: only recombinant monoclonal antibodies are used in our matched antibody pairs.

    • Specificity: antibody pairs are screened in plasma and serum to ensure specificity in complex samples.

    • Sensitivity: benchmarked against commercially available antibody pairs to ensure equivalent or superior performance compared with the competition.


     


    Additional buffers and plates are required for the assay. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).


     


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA® (ab218269) which uses the same antibody pair.


     


    To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 2 x 96 tests
    Human MASPIN Capture Antibody 1 x 100µg 1 x 20µg
    Human MASPIN Detector Antibody 1 x 25µg 1 x 5µg
    Human MASPIN Lyophilized Protein 1 vial 1 vial
  • Research areas

  • Function

    Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
  • Tissue specificity

    Normal mammary epithelial cells.
  • Sequence similarities

    Belongs to the serpin family. Ov-serpin subfamily.
  • Cellular localization

    Secreted > extracellular space.
  • Information by UniProt
  • Alternative names

    • Maspin
    • Ovalbumin
    • Peptidase inhibitor 5
    • PI-5
    • PI5
    • Protease inhibitor 5
    • protease inhibitor 5 (maspin)
    • Serine (or cysteine) peptidase inhibitor, clade B, member 5
    • serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5
    • Serpin B5
    • serpin family B member 5
    • Serpin peptidase inhibitor
    • Serpin peptidase inhibitor, clade B (ovalbumin), member 5
    • Serpinb5
    • SPB5_HUMAN
    • Spi5
    • Spi7
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab219539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Images

  • Standard calibration curve. Background subtracted values are graphed.

Protocols

References

ab219539 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab219539.
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