Human MBL ELISA Kit (Mannose-Binding Lectin) (ab193709)

Overview

  • Product name

    Human MBL ELISA Kit (Mannose-Binding Lectin)
    See all Mannan Binding Lectin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.03 ng/ml
  • Range

    0.03 ng/ml - 25 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 122.8 103% - 147%
    Plasma 121.1 98% - 145%
    Cell culture media 103.1 91% - 116%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s MBL (Mannose-Binding Lectin) Human ELISA Kit (ab193709) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MBL in sera, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human MBL coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures MBL present in the samples. The wells are washed and biotinylated anti-Human MBL antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of MBL bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    800X HRP-Streptavidin Concentrate 1 x 200µl
    Assay Diluent A 1 x 30ml
    Biotinylated Human MBL detection antibody (lyophilized) 2 vials
    Human MBL Standards (lyophilized) 2 vials
    Pre-coated Human MBL Microplate (12 strips x 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Function

    Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA.
  • Tissue specificity

    Plasma protein produced mainly in the liver.
  • Sequence similarities

    Contains 1 C-type lectin domain.
    Contains 1 collagen-like domain.
  • Domain

    The coiled-coil domain mediates trimerization.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • Collectin-1
    • Mannan-binding protein
    • Mannose-binding lectin
    • Mannose-binding protein C
    • MBL2
    • MBL2_HUMAN
    • MBP-C
    • MBP1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab193709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

  • Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Protocols

References

ab193709 has not yet been referenced specifically in any publications.

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