Overview

  • Product name

    Human MBL Oligomer ELISA Kit
    See all Mannan Binding Lectin/MBL kits
  • Detection method

    Colorimetric
  • Sample type

    Serum, Hep Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    = 0.02 ng/ml
  • Range

    0.5 ng/ml - 40 ng/ml
  • Assay time

    4h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Human MBL Oligomer ELISA Kit (ab207922) is an in vitro determination of oligomerized mannan-binding lectin in human serum or heparin plasma. For research use only.


    The assay is an ELISA performed in microwells coated with a monoclonal antibody against the MBL carbohydrate-binding domain. Bound MBL is detected with the same antibody that has been labeled with biotin, followed by development with horseradish peroxidase (HRP)-conjugated streptavidin and incubation with a chromogenic substrate. Comparison of the assay results with molecular size chromatography of MBL immunoreactivity in individual human serum samples suggests that the monoclonal antibody used is selective for MBL oligomers when used as both capture and detection antibody.

  • Notes

    Mannan-binding lectin (MBL, also called mannose binding lectin or mannose binding protein) is a multimeric carbohydrate-binding protein produced in the liver and secreted into the blood, where it constitutes an important element in innate immune defense against invading microorganisms. Its normally oligomerized forms are associated with specific serine proproteases (The MASPs) which are activated when MBL binds to microbial carbohydrate surfaces and in turn activate complement via the MBL or lectin pathway.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    12 x 8 coated Microwells + Frame 1 x 96 tests
    25x Wash Buffer Solution 1 x 30ml
    Biotinylated MBL Antibody 1 x 12ml
    HRP-Streptavidin Solution 1 x 12ml
    MBL Standard (0 ng/mL) 1 x 1ml
    MBL Standard (0.5 ng/mL) 1 x 1ml
    MBL Standard (1 ng/mL) 1 x 1ml
    MBL Standard (2 ng/mL) 1 x 1ml
    MBL Standard (5 ng/mL) 1 x 1ml
    MBL Standard (10 ng/mL) 1 x 1ml
    MBL Standard (20 ng/mL) 1 x 1ml
    MBL Standard (40 ng/mL) 1 x 1ml
    Sample Diluent Buffer 1 x 60ml
    Stop Solution 1 x 16ml
    TMB Substrate Solution 1 x 12ml
  • Research areas

  • Function

    Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA.
  • Tissue specificity

    Plasma protein produced mainly in the liver.
  • Involvement in disease

    Note=There is an association between low levels of MBL2 and a defect of opsonization which results in susceptibility to frequent and chronic infections.
  • Sequence similarities

    Contains 1 C-type lectin domain.
    Contains 1 collagen-like domain.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • COLEC 1
    • COLEC1
    • Collectin-1
    • HSMBPC
    • Lectin, mannose-binding, soluble, 2
    • Mannan binding lectin
    • Mannan binding protein
    • Mannan-binding protein
    • Mannose binding lectin
    • Mannose binding lectin (protein C) 2 soluble
    • Mannose binding lectin (protein C) 2, soluble
    • Mannose binding lectin (protein C) 2, soluble (opsonic defect)
    • Mannose binding lectin 2 soluble
    • Mannose binding lectin 2, soluble (opsonic defect)
    • Mannose binding lectin protein C2 soluble opsonic defect
    • Mannose binding protein
    • Mannose binding protein C
    • Mannose binding protein C precursor
    • Mannose binding protein, serum
    • Mannose-binding lectin
    • Mannose-binding protein C
    • MBL
    • MBL 2
    • MBL2
    • MBL2_HUMAN
    • MBL2D
    • MBP
    • MBP 1
    • MBP C
    • MBP-C
    • MBP1
    • MBPB
    • MBPC
    • MBPD
    • MGC116832
    • MGC116833
    • Opsonic defect
    • protein C
    • Soluble mannose binding lectin
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab207922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human MBL Oligomer ELISA Kit (ab207922) Calibration Curve

Protocols

References

ab207922 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab207922.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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