Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.26 pg/ml
- Range: 4.7 pg/ml - 300 pg/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Cit plasma, EDTA Plasma, Hep Plasma, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman MCP-1 ELISA Kit
See all MCP1 kits
Intra-assay Sample n Mean SD CV% Overall 5 2.5% Inter-assay Sample n Mean SD CV% Overall 3 6.7%
Sample typeCell culture supernatant, Urine, Serum, Plasma, Hep Plasma, EDTA Plasma, Cit plasma, Cerebral Spinal Fluid
Assay typeSandwich (quantitative)
Range4.7 pg/ml - 300 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 98 97% - 99% Urine 92 90% - 94% Serum 97 94% - 101% Hep Plasma 91 91% - % EDTA Plasma 94 90% - 97% Cit plasma 92 91% - 95% Cerebral Spinal Fluid 105 102% - 110%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Human
Does not react with: Cow
Human MCP1 (CCL2) ELISA kit (ab179886) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MCP1 protein in human serum, plasma, urine, and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate human MCP1 with 1.26 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.
ASSAY SPECIFICITY This kit recognizes both native and recombinant human MCP1 protein in serum, plasma, urine, and cell culture supernatant samples only. Cell and tissue extract samples have not been tested with this kit.
SPECIES REACTIVITY This kit recognizes human MCP1 protein.
Other species reactivity was determined by measuring MCP1 (100%) serum samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum assayed at the same dilution.
Reactivity < 3% was determined for the following species: Mouse and cow.
CROSS REACTIVITY Recombinant mouse MCP1 and rat MCP1 were prepared at 100 pg/mL and 50 pg/mL and assayed for cross reactivity. Cross-reactivity was only observed at 50 ng/mL.
This immunoassay is calibrated against a highly purified human MCP1. The NIBSC/WHO unclassified purified human MCP1 preparation 92/794 was evaluated in this kit.
The dose response curve of the unclassified standard MCP1 parallels the SimpleStep standard curve. To convert sample values obtained with the SimpleStep human MCP1 kit to approximate NIBSC 92/794 units, use the equation below.
NIBSC (92/794) approximate value (U/mL) = 0.000266 x SimpleStep human MCP1 value (pg/mL).
MCP-1 (CCL2) is a chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Human MCP-1 Capture Antibody 1 x 600µl 10X Human MCP-1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human MCP-1 Lyophilized Recombinant Protein (ab9670) 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
FunctionChemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
modificationsProcessing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
- Information by UniProt
- C-C motif chemokine 2
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
The MCP1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Example of human MCP1 standard curve in Sample Diluent NS. The MCP1 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
The concentrations of MCP1 were measured in duplicates, interpolated from the MCP1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 25%, plasma (heparin), 25%, and plasma (EDTA). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MCP1 concentration was determined to be 72 pg/mL in serum, 96 pg/mL in plasma (citrate), 101 pg/mL in plasma (heparin), and 88 pg/mL plasma (EDTA).
The concentrations of MCP1 were measured in duplicates, interpolated from the MCP1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cerebrospinal fluid (50%)), urine 100%, and PHA stimulated PBMC supernatant 0.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MCP1 concentration was determined to be 371 pg/mL in cerebrospinal fluid, 144 pg/mL in urine, and 70217 pg/mL in PHA stimulated PBMC supernatant.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MCP1 concentration was determined to be 113 pg/mL with a range of 58 - 247 pg/mL.
Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native MCP1 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
To learn more about the advantages of recombinant antibodies see here.
ab179886 has been referenced in 8 publications.
- Zhang H et al. Long noncoding RNA MIAT2 alleviates lipopolysaccharide-induced inflammatory damage in WI-38 cells by sponging microRNA-15. J Cell Physiol 235:3690-3697 (2020). PubMed: 31566734
- Hayatbakhsh MM et al. The Serum Levels of CCL2 and CCL16 Expression in Patients with Irritable Bowel Syndrome. Rep Biochem Mol Biol 8:9-14 (2019). PubMed: 31334281
- Sun H et al. Role of the mTOR-FOXO1 pathway in obesity-associated renal tubulointerstitial inflammation. Mol Med Rep 19:1284-1293 (2019). PubMed: 30535458
- Hosseinkhani B et al. Extracellular Vesicles Work as a Functional Inflammatory Mediator Between Vascular Endothelial Cells and Immune Cells. Front Immunol 9:1789 (2018). PubMed: 30131806
- Chen X et al. Dental Follicle Stem Cells Ameliorate Lipopolysaccharide-Induced Inflammation by Secreting TGF-ß3 and TSP-1 to Elicit Macrophage M2 Polarization. Cell Physiol Biochem 51:2290-2308 (2018). PubMed: 30537736
- Yamamoto M et al. Stage classification of IgG4-related dacryoadenitis and sialadenitis by the serum cytokine environment. Mod Rheumatol N/A:1-5 (2018). PubMed: 29385874
- Yu S et al. Mediating the invasion of smooth muscle cells into a cell-responsive hydrogel under the existence of immune cells. Biomaterials 180:193-205 (2018). PubMed: 30048909
- Jensen MR et al. Higher vascular endothelial growth factor-C concentration in plasma is associated with increased forearm capillary filtration capacity in breast cancer-related lymphedema. Physiol Rep 3:N/A (2015). ELISA ; Human . PubMed: 26059032