• Product name
    Human MCP1 ELISA Kit
    See all MCP1 kits
  • Detection method
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 2 pg/ml
  • Range
    2 pg/ml - 500 pg/ml
  • Recovery

    93 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 97.62 85% - 104%
    Serum 92.65 80% - 102%
    Plasma 90.42 79% - 101%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Product overview

    Abcam’s MCP1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MCP1 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human MCP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and MCP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MCP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    Get higher sensitivity in only 90 minutes with Human MCP1 ELISA Kit (CCL2) (ab179886) from our SimpleStep ELISA® range.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform



Our Abpromise guarantee covers the use of ab100586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Human MCP1 measured in biological fluids showing quantity (pg) per mL of tested sample. Samples diluted 2-10 fold.

  • Human MCP1 standard curve: mean of duplicates (+/- SD)
  • Human MCP1 measured in biological fluids showing quantity (pg) per mL of tested sample.
  • Representative Standard Curve using ab100586



ab100586 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Please note this product is unpublished so when you decide to order you need to contact our customer service team for them to put this item in your order.

I hope this info will help. Please let me know if you experience any difficulties.

This product is available but not published on our website. You cannot purchase this using our normal ordering system however I can add this product to your next shipment. Could you confirm how many vials customer would need..6?.
In my last I made...

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Thank you for contacting us. I apologize for the delay.

We are ready to publish this product. The product would be available at US dollar 120 per 200ul. The customer would need 6 vials of product so it will cost them $720 excluding shipping c...

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Thank you for your email. I am sorry for the delay while I was in contact with lab.

The volume of HRP-Streptavidin is very small and therefore would be difficult to see with the naked eye. The HRP-streptavidin volume sent was 8ul and is very ...

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Thank you for contacting us. Here is the requested information: ab46087: Normal values for TNFa in such samples are negatives or very low, so we usually recommend to assay the human serum samples neat with Standard buffer, human serum to dilute t...

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Thank you for your inquiry.  Below are the plasma values that we have measure during our QC tests with the mentioned ELISA kits: ab100575 : Ranging from undetectable to ~100pg/ml ab100586 : Ranging from undetectable to ~400pg/ml I hope this ...

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Thank you for contacting us. Unfortunately the ELISA assays have not been used in conjunction with a DSX analyser (and we therefore do not have any specific protocols for this) but as long as the machine can accurately carry out the protocol as stated ...

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