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Hello, I was wondering if you happened to have a protocol for running the MCP-1 and MCP-3 ELISA assays on a DSX analyzer. It looks as though it can be done, but I have concerns regarding the reagent dilutions, and would rather have a protocol that has been tested. We have a customer that is hoping to run your assay alongside ours for a study and we are trying to help them out. Also, how important is the "cover well" portion of the directions prior to the 2.5 hour incubation. Does it just need to be protected from light, or do the plate wells need to be sealed. If doing the 2.5 hour incubation, does it need to be shaken, or is that only with the overnight incubation in the refrigerator? Thank you,
Asked on Oct 17 2011
Thank you for contacting us. Unfortunately the ELISA assays have not been used in conjunction with a DSX analyser (and we therefore do not have any specific protocols for this) but as long as the machine can accurately carry out the protocol as stated in the manual we can see no reason why it would not be compatible. The "cover well" directions prior to incubation are there to avoid contamination and evaporation of the reagents. The only incubation that is light-sensitive is the TMB incubation. Additionally we recommend performing the incubations under gentle shaking/rocking (regardless of temperature or time). I hope this information has been helpful to you. If you have any further questions please do not hesitate to contact us.
Answered on Oct 17 2011