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What serum dilutions should be used with these 4 kits? uses human serum samples
Asked on Dec 01 2011
Thank you for contacting us. Here is the requested information: ab46087: Normal values for TNFa in such samples are negatives or very low, so we usually recommend to assay the human serum samples neat with Standard buffer, human serum to dilute the standard curve, or at the 1:2 dilution with standard buffer diluent PBS BSA (in that case, the standard curve should be diluted in same buffer). Below are our recommended normal human serum dilutions for the other 3 kits. These recommendations should only be used as a starting point and the optimal sample dilution needs to be determined by the enduser empirically based on researched literature and knowledge of their particular sample. ab100586: 2 fold dilution ab100610: 500-2000 fold dilution ab100640: 5-200 fold dilution I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Dec 01 2011