• Product name
    Human MCP3 ELISA Kit
    See all MCP3 kits
  • Detection method
  • Sample type
    Cell culture supernatant, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 20 pg/ml
  • Range
    24.58 pg/ml - 6000 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 88.99 79% - 98%
    Plasma 95.19 88% - 106%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s MCP3 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human MCP3 in plasma, and cell culture supernatants. (Human MCP3 concentration is pretty low in normal plasma, it may not be detected in this assay).

    This assay employs an antibody specific for Human MCP3 coated on a 96-well plate. Standards and samples are pipetted into the wells and MCP3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MCP3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MCP3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    We have not been able to detect endogenous Human MCP3 in normal serum with ab100588, only in serum spiked with Human MCP3.

    Get higher sensitivity in only 90 minutes with Human MCP3 ELISA Kit (CCL7) (ab193769) from our SimpleStep ELISA® range.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    240X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human MCP3 2 vials
    MCP3 Microplate (12 x 8 wells) 1 unit
    Recombinant Human MCP3 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Function
    Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3.
  • Sequence similarities
    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
  • Cellular localization
  • Information by UniProt
  • Alternative names
    • C-C motif chemokine 7
    • Ccl7
    • CCL7_HUMAN
    • Chemokine CC Motif Ligand 7
    • Chemotactic protein 3
    • FIC
    • Intercrine/chemokine MARC
    • MARC
    • MCP 3
    • MCP-3
    • Monocyte chemoattractant protein 3
    • Monocyte chemotactic protein 3
    • NC28
    • RANTES/sis homolog
    • RP23-350G1.4
    • SCYA6
    • SCYA7
    • Small inducible cytokine A7
    • Small-inducible cytokine A7
    see all
  • Database links

Associated products


Our Abpromise guarantee covers the use of ab100588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative Standard Curve Using ab100588.

  • Representative Standard Curve Using ab100588.



ab100588 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us. Unfortunately the ELISA assays have not been used in conjunction with a DSX analyser (and we therefore do not have any specific protocols for this) but as long as the machine can accurately carry out the protocol as stated ...

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