Description

  • Product name

    Human MDC1 (phospho S964) peptide
    See all MDC1 proteins and peptides
  • Purity

    70 - 90% by HPLC.

  • Animal free

    No
  • Nature

    Synthetic
    • Species

      Human
    • Modifications

      phospho S964

Associated products

Specifications

Our Abpromise guarantee covers the use of ab36491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-MDC1 (phospho S964) antibody (ab36017)

  • Form

    Liquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names

    • Homologue to Drosophila photoreceptor protein calphotin
    • MDC 1
    • Mdc1
    • MDC1_HUMAN
    • Mediation of DNA damage checkpoint 1
    • Mediator of DNA damage checkpoint 1
    • Mediator of DNA damage checkpoint protein 1
    • NFBD 1
    • NFBD1
    • Nuclear factor with BRCT domains 1
    • Nuclear Factor with BRCT Domains Protein 1
    see all
  • Function

    Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1/CHK1 and CHEK2/CHK2/CDS1 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.
  • Tissue specificity

    Highly expressed in testis.
  • Sequence similarities

    Contains 2 BRCT domains.
    Contains 1 FHA domain.
  • Domain

    Tandemly repeated BRCT domains are characteristic of proteins involved in DNA damage signaling. In MDC1, these repeats are required for localization to chromatin which flanks sites of DNA damage marked by 'Ser-139' phosphorylation of H2AFX.
  • Post-translational
    modifications

    Phosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint.
  • Cellular localization

    Nucleus. Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires 'Ser-139' phosphorylation of H2AFX. Colocalizes with APTX at sites of DNA double-strand breaks.
  • Information by UniProt

Images

  • All lanes : Anti-MDC1 (phospho S964) antibody (ab36017) at 1/250 dilution

    Lane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate with Human MDC1 (phospho S964) peptide (ab36491) at 1/250 dilution
    Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate with Human MDC1 peptide (ab36492) at 1/250 dilution

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lane 1 : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Lanes 2-3 : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Exposure time: 20 minutes

References

ab36491 has not yet been referenced specifically in any publications.

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