Key features and details
- Suitable for: Blocking
Product nameHuman MDC1 (phospho S964) peptide
See all MDC1 proteins and peptides
Purity70 - 90% by HPLC.
Our Abpromise guarantee covers the use of ab36491 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-MDC1 (phospho S964) antibody (ab36017)
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
- Homologue to Drosophila photoreceptor protein calphotin
- MDC 1
FunctionRequired for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1/CHK1 and CHEK2/CHK2/CDS1 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.
Tissue specificityHighly expressed in testis.
Sequence similaritiesContains 2 BRCT domains.
Contains 1 FHA domain.
DomainTandemly repeated BRCT domains are characteristic of proteins involved in DNA damage signaling. In MDC1, these repeats are required for localization to chromatin which flanks sites of DNA damage marked by 'Ser-139' phosphorylation of H2AFX.
modificationsPhosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint.
Cellular localizationNucleus. Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires 'Ser-139' phosphorylation of H2AFX. Colocalizes with APTX at sites of DNA double-strand breaks.
- Information by UniProt
All lanes : Anti-MDC1 (phospho S964) antibody (ab36017) at 1/250 dilution
Lane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate with
Human MDC1 (phospho S964) peptide (ab36491) at 1/250 dilution
Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate with Human MDC1 peptide (ab36492) at 1/250 dilution
Lysates/proteins at 10 µg per lane.
Lane 1 : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Lanes 2-3 : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Exposure time: 20 minutes
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab36491 has not yet been referenced specifically in any publications.