Product nameHuman MDFIC peptide
Purity70 - 90% by HPLC.
Our Abpromise guarantee covers the use of ab86656 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
- Human I mfa domain containing protein
- Human I-mfa domain-Containing protein
RelevanceMDFIC is a cellular factor capable of modulating the expression of cellular and viral promoters. Its synthesis is controlled at the translational level by two different codons, an ATG and an upstream non-ATG translational initiator, allowing the production of two protein isoforms that present different subcellular localizations, p32 (isoform 2) being mainly distributed throughout the cytoplasm, whereas p40 (isoform 1) is targeted to the nucleolus. MDFIC is known to down-regulate Tat-dependent transcription of HIV-1 LTR by interacting with HIV-1 Tat and Rev proteins and impairing their nuclear import (possibly by rendering the NLS domains inaccessible to importin-beta) and stimulate activation of human T-cell leukaemia virus type I (HTLV-I) LTR. In human cells, MDFIC binds to the axin complex, resulting in an increase in the level of free beta-catenin; it affects axin regulation of the WNT and JNK signalling pathways.
Cellular localizationCytoplasmic and Nuclear
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab86656 has not yet been referenced specifically in any publications.