Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Product nameHuman Anti-Measles virus IgG ELISA Kit
See all Measles Virus IgG kits
Intra-assay Sample n Mean SD CV% Pos. Serum 18 9.4% Pos. Serum 24 3.2% Inter-assay Sample n Mean SD CV% Pos. Serum 12 5% Pos. Serum 12 1.5%
Sample typeSerum, Hep Plasma, Cit plasma
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s anti-Measles virus IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Measles virus in Human serum and plasma.
A 96-well plate has been precoated with Measles virus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Measles virus-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Measles virus IgG sample captured in plate.
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover foil 1 unit IgG Sample Diluent Yellow, White cap 1 x 100ml Measles IgG Cut-off Control Yelow, Green cap 1 x 3ml Measles IgG Negative Control Yellow, Blue cap 1 x 2ml Measles IgG Positive Control Yellow, Red cap 1 x 2ml Measles virus (IgG) Coated Microplate (12 x 8 wells) 1 unit Measles virus anti-IgG HRP Conjugate Blue, Black cap 1 x 20ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml
ab108750 has been referenced in 1 publication.
- Ciencewicki JM et al. Plasma Donors in the Southwestern United States Positively Contribute to the Diverse Therapeutic Antibody Profile of Immune Globulin Products. Sci Rep 10:6850 (2020). PubMed: 32321978